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Multifunctional alginate nanoparticles containing nitric oxide donor and silver nanoparticles for biomedical applications.
Biomaterials Advances ( IF 7.9 ) Pub Date : 2020-04-08 , DOI: 10.1016/j.msec.2020.110933
Alessandro L Urzedo 1 , Marcelly C Gonçalves 2 , Mônica H M Nascimento 1 , Christiane B Lombello 3 , Gerson Nakazato 2 , Amedea B Seabra 1
Affiliation  

Nitric oxide (NO) is an endogenous molecule with antimicrobial activity. Silver nanoparticles (AgNPs) are also well known for its antimicrobial properties. In this work, we prepared, characterized, evaluated the cytotoxicity and the antibacterial activity of alginate nanoparticles (alginate NPs) containing S-nitroso-mercaptosuccinic acid (S-nitroso-MSA, a NO donor) and/or AgNPs. AgNPs were obtained by plant mediated synthesis using green tea (Camellia sinensis), according to the green principles. Alginate NPs were obtained by ionotropic gelation with calcium ions (Ca2+) and used to incorporate the NO donor and/or AgNPs. The obtained nanoparticles containing S-nitroso-MSA and/or AgNPs have hydrodynamic size distribution in the range of 103.3 ± 2.9–414.2 ± 22.6 nm with moderate polydispersity index and negative zeta potentials. Kinetic measurements showed a sustained NO release from alginate NPs, at the mili molar range. Both S-nitroso-MSA and AgNPs were able to diffuse from the nanoparticles following a Fickian diffusion mathematical model. The cytotoxicity of the nanoparticles was evaluated towards Vero cells, and a concentration dependent toxicity was observed. The combination of the NO donor and green tea AgNPs enhanced the toxicity to the tested cells. Finally, the antibacterial activity of the nanoparticles was demonstrated against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, and Streptococcus mutans UA159. Interestingly, the combination of the NO donor and AgNPs into alginate NPs had a superior antibacterial activity, compared with bacteria treated with alginate NPs containing S-nitroso-MSA or AgNPs individually. Moreover, antibacterial effects were observed at nanoparticle concentrations not toxic to Vero cell. To the best of knowledge, this is the first report to demonstrate the ability of alginate NPs releasing NO and AgNPs with potent antimicrobial activity.



中文翻译:

包含一氧化氮供体和银纳米颗粒的多功能藻酸盐纳米颗粒,用于生物医学应用。

一氧化氮(NO)是具有抗菌活性的内源性分子。银纳米颗粒(AgNPs)也因其抗菌特性而闻名。在这项工作中,我们准备,表征,评估了含有S-亚硝基巯基琥珀酸(S-亚硝基-MSA,NO供体)和/或AgNPs的藻酸盐纳米颗粒(藻酸盐NP)的细胞毒性和抗菌活性。根据绿色原理,使用绿茶(山茶)通过植物介导的合成获得AgNP 。通过与钙离子(Ca 2+)并用于合并NO供体和/或AgNP。所获得的含有S-亚硝基-MSA和/或AgNPs的纳米颗粒的流体力学尺寸分布在103.3±2.9–414.2±22.6 nm范围内,具有中等的多分散指数和zeta负电势。动力学测量表明,在小摩尔范围内,藻酸盐NP持续释放NO。遵循Fickian扩散数学模型,S-亚硝基-MSA和AgNPs都能从纳米颗粒扩散。评价了纳米颗粒对Vero细胞的细胞毒性,并观察到浓度依赖性毒性。NO供体和绿茶AgNPs的组合增强了对测试细胞的毒性。最后,证明了纳米颗粒对大肠杆菌ATCC 25922的抗菌活性,金黄色葡萄球菌ATCC 25923和变形链球菌UA159。有趣的是,与单独使用含S-亚硝基-MSA或AgNP的藻酸盐NP处理的细菌相比,将NO供体和AgNP组合成藻酸盐NP具有更好的抗菌活性。此外,在对Vero细胞无毒的纳米颗粒浓度下观察到了抗菌作用。就其所知,这是第一份证明藻酸盐NP释放具有强大抗菌活性的NO和AgNP的能力的报告。

更新日期:2020-04-08
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