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Fungal In Situ Assembly Gives Novel Properties to CdSxSe1–x Quantum Dots for Sensitive Label-Free Detection of Chloramphenicol
ACS Sustainable Chemistry & Engineering ( IF 8.4 ) Pub Date : 2020-04-08 , DOI: 10.1021/acssuschemeng.0c01698
Xiaoguang Xu 1 , Ying Yang 2 , Han Jin 1 , Bing Pang 1 , Rongrong Yang 1 , Lu Yan 1 , Chunmei Jiang 1 , Dongyan Shao 1 , Junling Shi 1
Affiliation  

Quantum dots (QDs) are attracting significant attention for the development of sensitive detection methods because of their unique optical properties. Biosynthetic QDs using organisms is a promising alternative route to chemical synthesis because it is a simple and eco-friendly process. Herein, we developed a straightforward and green system for the biological assembly of CdSxSe1–x QDs by Phomopsis sp. XP-8 within 6 h, a more efficient approach than those reported in other organisms. The QDs were shown to have a CdS0.75Se0.25@oligopeptide transporter structure, and the production process was shown to be strongly influenced by intracellular glutathione content. The QDs were monodispersed with a uniform spherical shape of 3.22 ± 0.07 nm in diameter. They exhibited good water solubility and excellent fluorescence properties. The QDs could be extracted and used directly as a sensitive chloramphenicol (CAP) probe via static fluorescence quenching in the linear range from 3.13 to 500 μg/L with a detection limit of 0.89 μg/L. The detection method was highly selective for CAP with minimal interference from other antibiotics and was used to successfully detect CAP in milk samples. Overall, this work has great significance for the development of a fast and simple QD synthesis system via biological assembly.

中文翻译:

真菌原位组装赋予CdS x Se 1– x量子点新颖的特性,可用于氯霉素的无标签灵敏检测

由于其独特的光学特性,量子点(QD)引起了灵敏检测方法的发展的极大关注。使用生物体进行生物合成量子点是化学合成的一种有前途的替代途径,因为它是一种简单且环保的方法。在这里,我们开发了一种简单而绿色的系统,用于Phomopsis sp。的CdS x Se 1– x QD的生物组装。XP-8在6小时之内,比其他生物报告的方法更为有效。量子点显示CdS 0.75 Se 0.25寡肽转运蛋白的结构和生产过程受到细胞内谷胱甘肽含量的强烈影响。QD被单分散为直径为3.22±0.07 nm的均匀球形。它们表现出良好的水溶性和优异的荧光性能。可以提取QD,并将其通过3.13至500μg/ L的线性范围内的静态荧光猝灭直接用作灵敏的氯霉素(CAP)探针,检测极限为0.89μg/ L。该检测方法对CAP具有高度选择性,并且不受其他抗生素的干扰最小,可用于成功检测牛奶样品中的CAP。总体而言,这项工作对于通过生物组装开发快速简便的QD合成系统具有重要意义。
更新日期:2020-04-08
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