Because nitric oxide (NO) gas is an endogenously produced signaling molecule related to numerous physiological functions, manystudies have been conducted to develop NO delivery systems for potential biomedical applications. However, NO is a reactive radical gas molecule that has a very short life-time and readily transforms into nitrogen oxide species via reaction with oxygen species. Therefore, it is necessary to develop an NO delivery carrier that allows local release of the NO gas at the site of application. In this study, Laponite (LP) nanoclay was used to fabricate an NO delivery carrier through the formation of Laponite–polyamine (LP–PAn) composites. The Laponite clay and pentaethylenehexamine (PEHA) formed a macromolecular structure by electrostatic interaction and the nitric oxide donor, N-diazeniumdiolate (NONOates), was synthesized into the LP–PAn composite. We investigated the conformation of the LP–PAn composite structure and the NO donor formation by ζ potential, X-ray diffraction, and UV–vis and Fourier transform infrared (FT-IR) spectroscopies and also by analyzing the NO release profile. Additionally, we confirmed the applicability in biomedical applications via a cell viability and in vitro endothelial cell tube formation assay.

中文翻译：

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纳米粘土-多胺复合水凝胶通过皂石的固有胶凝特性局部输送一氧化氮气体。

由于一氧化氮（NO）气体是内源性产生的与许多生理功能相关的信号分子，因此进行了许多研究以开发用于潜在生物医学应用的NO输送系统。但是，NO是一种活性自由基气体分子，具有很短的使用寿命，并且很容易通过与氧的反应转化为氮氧化物。因此，有必要开发一种NO输送载体，其允许在应用部位局部释放NO气体。在这项研究中，Laponite（LP）纳米粘土用于通过形成Laponite-多胺（LP-PA n）复合材料来制造NO输送载体。Laponite粘土和五亚乙基六胺（PEHA）通过静电相互作用和一氧化氮供体N形成了大分子结构-重氮二醇二甲酸酯（NONOates）被合成为LP-PA n复合材料。我们通过ζ电位，X射线衍射，UV-vis和傅里叶变换红外（FT-IR）光谱学以及通过分析NO释放曲线，研究了LP-PA n复合结构的构型和NO供体的形成。此外，我们通过细胞生存力和体外内皮细胞管形成试验证实了在生物医学应用中的适用性。