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LncRNA DLEU2 promotes tumour growth by sponging miR‐337‐3p in human osteosarcoma
CELL BIOCHEMISTRY AND FUNCTION ( IF 3.6 ) Pub Date : 2020-03-20 , DOI: 10.1002/cbf.3509
Wei Liu 1 , Peng‐cheng Liu 2 , Ke Ma 3 , Yuan‐yi Wang 1 , Qing‐bao Chi 1 , Ming Yan 1
Affiliation  

According to statistics, abnormal regulation of lncRNAs pivotally influences multiple malignant tumours. DLEU2, as one of these lncRNAs, is detected to be related to growth and development of tumours. The molecular mechanisms of DLEU2 in osteosarcoma, however, are still unknown. QRT‐PCR was adopted to analyse the correlations of clinicopathological features and prognosis of osteosarcoma cases with DLEU2. The influences of DLEU2 on cell migration and viability were evaluated independently by experiments in vitro and in vivo. Bioinformatics analysis, RNA immunoprecipitation (RIP) assay, and dual luciferase reporter gene assay confirmed the specific binding of DLEU2 to miR‐337‐3p. Moreover, rescue experiments were carried out to further evaluate the regulatory association between miR‐337‐3p expression and DLEU2. In osteosarcoma tissues and cells, DLEU2 expression level was raised remarkably in comparison with that in para‐carcinoma normal tissues, and DLEU2 high expression had associations with poor prognosis, tumour stages, and TS of osteosarcoma cases. Cell migration ability and viability were blocked by DLEU2 knockdown but enhanced by ectopic DLEU2 expression in vitro and in vivo. Additionally, DLEU2 was found to sponge miR‐337‐3p and trigger the stimulating effect in osteosarcoma cells, which would be suppressed by miR‐337‐3p mimics. Furthermore, a negative correlation existed between miR‐337‐3p expression and DLEU2 in osteosarcoma tissues. This study manifests that DLEU2 sponges miR‐337‐3p to accelerate tumour growth and is confirmed to be a factor for poor prognosis of osteosarcoma cases.

中文翻译:

LncRNA DLEU2通过使人骨肉瘤中的miR‐337-3p海绵化来促进肿瘤生长

据统计,lncRNA的异常调节关键地影响多个恶性肿瘤。DLEU2,作为这些lncRNA之一,被检测与肿瘤的生长和发展有关。然而,DLEU2在骨肉瘤中的分子机制仍然未知。采用QRT-PCR分析DLEU2与骨肉瘤的临床病理特征及预后的相关性。DLEU2对细胞迁移和活力的影响通过体外和体内实验独立评估。生物信息学分析,RNA免疫沉淀(RIP)分析和双重荧光素酶报告基因分析证实了DLEU2的特异性结合至miR‐337‐3p。此外,进行了拯救实验以进一步评估miR-337-3p表达与DLEU2之间的调节关联。与癌正常组织相比,在骨肉瘤组织和细胞中,DLEU2表达水平显着升高,并且DLEU2高表达与骨肉瘤病例的不良预后,肿瘤分期和TS相关。细胞迁移能力和生存能力被DLEU2敲低所阻断,但被异位DLEU2在体外和体内的表达所增强。此外,DLEU2被发现可以使miR-337-3p海绵化并触发骨肉瘤细胞的刺激作用,而miR-337-3p模仿物会抑制这种刺激作用。此外,骨肉瘤组织中miR-337-3p表达与DLEU2之间存在负相关。这项研究表明DLEU2使miR‐ 337‐3p海绵加速肿瘤生长,并被证实是骨肉瘤病例预后不良的因素。
更新日期:2020-03-20
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