LncRNA RP11‐363E7.4 has been shown to be downregulated in gastric cancer (GC), while the effect of lncRNA RP11‐363E7.4 on GC and its potential molecular mechanisms is unclear. The purpose of this study was to explore the functional role and underlying molecular mechanisms of lncRNA RP11‐363E7.4 involved in GC progress.To address the question, quantitative real‐time PCR assay was performed to confirm lncRNA RP11‐363E7.4 expression levels in GC tissues and cell lines. Cell proliferation, apoptosis, migration and invasion were estimated using Cell Counting Kit‐8, colony formation, scratch wound healing and Transwell assays. Potential molecular mechanisms were evaluated using western blot assay. The results showed that lncRNA RP11‐363E7.4 was significantly downregulated in GC cell lines and 82 paired tissues. The correlation between expression and clinicopathological features indicated that low expression of lncRNA RP11‐363E7.4 was associated with T stage (P = .010). Functional experiments showed that overexpression of lncRNA RP11‐363E7.4 prevented proliferation, migration, and invasion and induced apoptosis of GC cells. Western blot assay revealed that lncRNA RP11‐363E7.4 functioned via the p53, Bax/Bcl‐2, β‐catenin pathway. In summary, this study revealed that lncRNA RP11‐363E7.4 functioned as a tumour suppressor by inhibiting proliferation, migration, and invasion and inducing apoptosis of GC cells. Significance of the study:LncRNA RP11‐363E7.4 has been shown to be downregulated in GC, while the effect of lncRNA RP11‐363E7.4 on GC and its potential molecular mechanism is unclear. We revealed that lncRNA RP11‐363E7.4 functioned as a tumour suppressor by inhibiting proliferation, migration, and invasion and inducing apoptosis of GC cells. LncRNA RP11‐363E7.4 might become an attractive diagnostic and prognostic biomarker of GC and a promising target for GC treatment.

中文翻译：

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长非编码RNA RP11-363E7.4的过表达抑制胃癌的增殖和侵袭

LncRNA RP11-363E7.4在胃癌（GC）中已被下调，而lncRNA RP11-363E7.4对GC的作用及其潜在的分子机制尚不清楚。这项研究的目的是探讨lncRNA RP11-363E7.4参与胃癌进展的功能和潜在分子机制。为解决这个问题，进行了定量实时PCR分析以确认lncRNA RP11-363E7.4表达水平在GC组织和细胞系中。使用Cell Counting Kit-8，集落形成，刮伤愈合和Transwell分析评估细胞增殖，凋亡，迁移和侵袭。使用蛋白质印迹分析评估潜在的分子机制。结果表明，ncc RP11-363E7.4在GC细胞系和82对配对组织中显着下调。P = .010）。功能实验表明，lncRNA RP11-363E7.4的过度表达可阻止GC细胞的增殖，迁移和侵袭并诱导其凋亡。Western blot分析显示，lncRNA RP11-363E7.4通过p53，Bax / Bcl-2，β-catenin途径起作用。总而言之，这项研究表明，lncRNA RP11-363E7.4通过抑制GC细胞的增殖，迁移和侵袭并诱导其凋亡而起到抑癌作用。学习的重点LncRNA RP11-363E7.4已在GC中被下调，而lncRNA RP11-363E7.4对GC的作用及其潜在的分子机制尚不清楚。我们揭示了lncRNA RP11-363E7.4通过抑制GC细胞的增殖，迁移和侵袭并诱导其凋亡而起到抑癌作用。LncRNA RP11-363E7.4可能会成为有吸引力的GC诊断和预后生物标志物，并有望成为GC治疗的目标。