We used molecular tools and a multilocus approach to investigate the phylogeography of Lepidothrix coronata across most of its ample range. We sequenced six DNA fragments to produce phylogenies, molecular dating estimates, analyses of the dynamics of the demographic history of the species and a biogeographic analysis to estimate the events and changes in the ancestral distribution of the species. The results indicated the presence of four well‐established lineages, with high levels of divergence. These lineages are delineated by well‐defined geographic barriers, with one lineage, restricted to the west of the Andes, being the first to diverge from the complex. The other three lineages are exclusive to the Amazonian distribution of the species, with two being found north of the Amazon River, and the third, south of the Amazon. Some of the relationships found between these lineages were distinct from those described in previous studies. Important disagreements were found between the mtDNA phylogeny and that of the multilocus analysis, in relation to the lineages located to the west of the Andes. We propose that past introgression events may have influenced shifts in the relationships between lineages, despite the fact that the groups were well defined in both the phylogenies. The biogeographic analysis indicates that the lineages arose through successive vicariance events, which had a primary role in the diversification of the group. Two or three genetically structured subclades were also found within each Amazonian lineage, although these subclades are not isolated by an obvious geographic barrier.

中文翻译：

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Manakin 物种的多位点数据揭示了由替代性塑造的神秘多样化

我们使用分子工具和多位点方法来研究 Lepidothrix coronata 在其大部分范围内的系统地理学。我们对六个 DNA 片段进行了测序，以产​​生系统发育、分子测年估计、物种人口历史动态分析和生物地理分析，以估计物种祖先分布的事件和变化。结果表明存在四个成熟的谱系，具有高度的分歧。这些世系由明确的地理障碍划定，其中一个世系仅限于安第斯山脉以西，是第一个从复合体分出的世系。其他三个谱系是该物种在亚马逊地区独有的，其中两个在亚马逊河以北，第三个在亚马逊以南。在这些谱系之间发现的一些关系与先前研究中描述的关系不同。关于位于安第斯山脉以西的谱系，在 mtDNA 系统发育和多位点分析之间发现了重要的分歧。我们提出过去的基因渗入事件可能影响了谱系之间关系的变化，尽管这些群体在两个系统发育中都有很好的定义。生物地理学分析表明，这些谱系是通过连续的替代事件产生的，这在该群体的多样化中起主要作用。在每个亚马逊血统中也发现了两个或三个基因结构的亚支，尽管这些亚支并没有被明显的地理障碍隔离。