The main effector mechanisms of neutrophils are the release of neutrophil extracellular traps (NETs) and myeloperoxidase (MPO). In this work, we evaluated the role of NETs and the activity of MPO in the interactions of rodent neutrophils with amoebae and in amoebic liver abscess (ALA)-resistant and ALA-susceptible models. We showed with in vitro assays that mice produced greater amounts of NETs and MPO than did hamsters, and the elastase activity was high in both models. However, the inhibition of NETs and MPO promoted an increase in amoeba viability in the mice. The mouse ALAs showed a more profound presence of NETs and MPO than did the hamster ALAs. We concluded that both effector mechanisms were essential for the amoebic damage and could prevent the formation of ALAs in the resistant model.

中文翻译：

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中性粒细胞胞外陷阱和MPO在对溶组织变形虫的敏感性和抗性模型中。

中性粒细胞的主要效应机制是中性粒细胞胞外捕获物（NETs）和髓过氧化物酶（MPO）的释放。在这项工作中，我们评估了NETs和MPO在啮齿动物嗜中性粒细胞与变形虫的相互作用以及对阿米巴抗肝脓肿（ALA）和ALA敏感的模型中的作用。我们通过体外试验显示，与仓鼠相比，小鼠产生的NET和MPO数量更多，并且两种模型中的弹性蛋白酶活性均很高。但是，NETs和MPO的抑制促进了小鼠变形虫活力的增加。与仓鼠ALA相比，小鼠ALA显示出更深层的NET和MPO。我们得出的结论是，两种效应子机制对于阿米巴损伤都是必不可少的，并且可以阻止耐药模型中ALA的形成。