Confocal Raman microspectroscopy is being increasingly used to investigate the skin penetration of actives and excipients. The aim of the present study was to compare results obtained with skin from different donors, acquired with two Raman systems: the RiverD gen2 Skin Composition Analyzer and the WiTec alpha 500 instrument. To elucidate the interindividual variability between the skin donors and to find out whether the two systems would give similar results in skin penetration analysis, we compared the penetration of the model drug procaine from hydrogel formulations with or without the penetration enhancer POE‐23‐lauryl ether. Penetration was investigated with both systems on skin from three different skin donors, each. Penetration plots were prepared by plotting the signal intensity of a typical peak of procaine against the skin depth. We then calculated the enhancement ratio from the areas under the curves of the penetration plots of procaine that had penetrated from the formulation with or without the enhancer. They ranged between 2.0 and 3.1 for the WiTec system and between 2.1 and 3.1 for the RiverD system. Thus, we found that both systems provide similar estimations of the skin penetration of procaine.

中文翻译：

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共聚焦拉曼光谱法测定皮肤渗透曲线：评估个体间变异性和实验室间可比性

共焦拉曼光谱法越来越多地用于研究活性物质和赋形剂的皮肤渗透性。本研究的目的是比较使用两种拉曼系统（RiverD gen2皮肤成分分析仪和WiTec alpha 500仪器）从不同供体的皮肤获得的结果。为了阐明皮肤供体之间的个体差异，并找出这两个系统在皮肤渗透分析中是否会给出相似的结果，我们比较了有或没有渗透促进剂POE-23-月桂基醚的水凝胶制剂中模型普鲁卡因的渗透率。用这两种系统对来自三个不同皮肤供体的皮肤的渗透性进行了研究。通过将普鲁卡因的典型峰的信号强度相对于皮肤深度作图来制备穿透图。然后，我们根据普鲁卡因的渗透曲线下的面积计算出了增强率，该普鲁卡因的渗透曲线从有或没有增强剂的制剂中渗透而来。对于WiTec系统，它们的范围介于2.0和3.1之间，对于RiverD系统，它们的范围介于2.1和3.1之间。因此，我们发现两个系统都对普鲁卡因的皮肤渗透提供了相似的估计。