Alcalase potato protein hydrolysate (APPH) might have a very important role in therapeutic effects. This study aims to examine the beneficial effects of bioactive peptides (DIKTNKPVIF [DI] and IF) from APPH supplement in the regulation of cardiac apoptosis, autophagy, and mitochondrial biogenesis pathway in spontaneously hypertensive rats (SHR). We have investigated ejection fraction, fractional shortening, Tunel assay, apoptosis, autophagy, and mitochondrial biogenesis pathway marker expression to show the efficacy of bioactive peptides in an SHR model. Bioactive peptides significantly upregulate ejection fraction and fractional shortening in SHR rats. SHR rats exhibited higher protein expression of apoptotic markers such as BAD, cytochrome c, and caspase 3. Finally, the bioactive peptides upregulate survival proteins (p‐AKT/p‐PI3K), autophagy (Beclin1/LC3B), and mitochondrial biogenesis (p‐AMPKα/SIRT1/PGC1α/p‐Foxo3a/Nrf2/CREB) marker expressions compared with the SHR groups. In summary, the bioactive peptides protect the heart tissues through the activation of autophagy and mitochondrial biogenesis pathway and thereby attenuate cardiac apoptosis in a spontaneously hypertensive rat model.

中文翻译：

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生物活性肽通过激活自噬和线粒体生物发生途径减轻自发性高血压大鼠心脏的心脏凋亡

Alcalase马铃薯蛋白水解物（APPH）可能在治疗效果中起非常重要的作用。本研究旨在检查来自 APPH 补充剂的生物活性肽（DIKTNKPVIF [DI] 和 IF）在调节自发性高血压大鼠 (SHR) 的心脏细胞凋亡、自噬和线粒体生物发生途径中的有益作用。我们研究了射血分数、缩短分数、Tunel 测定、细胞凋亡、自噬和线粒体生物发生途径标记表达，以显示生物活性肽在 SHR 模型中的功效。生物活性肽显着上调 SHR 大鼠的射血分数和缩短分数。SHR 大鼠表现出更高的凋亡标志物蛋白表达，如 BAD、细胞色素 c 和半胱天冬酶 3。最后，生物活性肽上调存活蛋白 (p-AKT/p-PI3K)，与 SHR 组相比，自噬 (Beclin1/LC3B) 和线粒体生物发生 (p-AMPKα/SIRT1/PGC1α/p-Foxo3a/Nrf2/CREB) 标记表达。总之，生物活性肽通过激活自噬和线粒体生物发生途径来保护心脏组织，从而减轻自发性高血压大鼠模型中的心脏细胞凋亡。