当前位置: X-MOL 学术Environ. Toxicol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Cardiac protection of Bauhinia championii against reperfusion injury
Environmental Toxicology ( IF 4.5 ) Pub Date : 2020-02-15 , DOI: 10.1002/tox.22912
Yun‐Fang Chen, Wei‐Yu Chen, Ching‐Hu Chung, Chao‐Lin Kuo, An‐Sheng Lee

This study aims to investigate the protective effects of the Bauhinia championii (BC) against ischemia/reperfusion (I/R)‐induced injury in an isolated heart model. Langendorff‐perfused C57BL/6JNarl mice hearts were performed with 30 minutes ischemia and 60 minutes reperfusion by left anterior descending artery ligation. Before reperfusion, boiling water extracts of BC (10 mg/L) was pretreated for 15 minutes. During reperfusion, BC significantly decreased the occurrence of ventricular arrhythmias by lead II electrocardiogram (ECG). Electrophysiological effect of BC was further determined in isolated ventricular myocytes by whole‐cell patch clamp technique. The underlying mechanism may result from its Na+ channel blocking activity characterized with reduced rise slope of action potential and Na+ current density. Moreover, BC dramatically reduced I/R‐caused infarct size, which was accessed by 2,3,5‐triphenyltetrazolium chloride (TTC) assay. Since BC decreased I/R‐induced myoglobin release and oxidation of Ca2+‐calmodulin‐dependent protein kinase, inhibition of myocardial necroptosis may account for the protective effects of BC on myocytes lose. This study indicated that BC may prevent I/R induced ventricular arrhythmias and myocyte death by blocking Na+ channels and decreasing necroptosis, respectively. Since most of the available antiarrhythmic remedies have unwanted adverse actions, BC could be a novel candidate for the treatment of myocardial infarction and ventricular arrhythmia.

中文翻译:

冠军紫荆抗再灌注损伤的心脏保护作用

本研究旨在研究紫荆花 (BC) 在离体心脏模型中对缺血/再灌注 (I/R) 诱导的损伤的保护作用。Langendorff 灌注的 C57BL/6JNarl 小鼠心脏通过左前降支结扎术进行 30 分钟缺血和 60 分钟再灌注。再灌注前,将 BC (10 mg/L) 的沸水提取物预处理 15 分钟。在再灌注期间,BC 通过 II 导联心电图 (ECG) 显着降低室性心律失常的发生。通过全细胞膜片钳技术在分离的心室肌细胞中进一步测定了 BC 的电生理效应。潜在机制可能源于其 Na+ 通道阻断活动,其特征是动作电位和 Na+ 电流密度的上升斜率降低。而且,BC 显着减少了 I/R 引起的梗塞面积,可通过 2,3,5-三苯基四唑氯化物 (TTC) 测定获得。由于 BC 减少了 I/R 诱导的肌红蛋白释放和 Ca2+-钙调蛋白依赖性蛋白激酶的氧化,因此抑制心肌坏死可能是 BC 对心肌细胞失去保护作用的原因。该研究表明,BC 可分别通过阻断 Na+ 通道和减少坏死性凋亡来预防 I/R 诱导的室性心律失常和心肌细胞死亡。由于大多数可用的抗心律失常药物都有不需要的不良作用,BC 可能是治疗心肌梗塞和室性心律失常的新候选药物。由于 BC 减少了 I/R 诱导的肌红蛋白释放和 Ca2+-钙调蛋白依赖性蛋白激酶的氧化,因此抑制心肌坏死可能是 BC 对心肌细胞失去保护作用的原因。该研究表明,BC 可分别通过阻断 Na+ 通道和减少坏死性凋亡来预防 I/R 诱导的室性心律失常和心肌细胞死亡。由于大多数可用的抗心律失常药物都有不需要的不良作用,BC 可能是治疗心肌梗塞和室性心律失常的新候选药物。由于 BC 减少了 I/R 诱导的肌红蛋白释放和 Ca2+-钙调蛋白依赖性蛋白激酶的氧化,因此抑制心肌坏死可能是 BC 对心肌细胞失去保护作用的原因。该研究表明,BC 可分别通过阻断 Na+ 通道和减少坏死性凋亡来预防 I/R 诱导的室性心律失常和心肌细胞死亡。由于大多数可用的抗心律失常药物都有不需要的不良作用,BC 可能是治疗心肌梗塞和室性心律失常的新候选药物。
更新日期:2020-02-15
down
wechat
bug