Oleochemicals have long been used as biolubricants, biopolymers, and biosurfactants; an effective alternative to petroleum‐based products. The present study explores the biosurfactant potential of a novel strain, isolated from rocks of earthquake‐prone area. On the basis of morphological, biochemical and 16S rRNA sequencing analysis, the isolate was identified as Staphylococcus nepalensis (KY024500). A biosurfactant yield 2.39, 1.39, and 0.9 g L⁻¹ was obtained using glycerol, waste orange peel, and diesel as a sole carbon source, respectively. Based on oil recovery experimental findings through sand pack column, the obtained biosurfactant from waste orange peels as a sole carbon source was carried forward for further analysis. Thus, obtained biosurfactant from waste orange peels were subjected to solvent extraction and purified by column chromatography. The purified biosurfactant thus obtained was characterized with the help of fourier transform infrared (FTIR), nuclear magnetic resonance (NMR), gas chromatography‐mass spectroscopy (GC–MS), and MALDI TOF MS/mass spectroscopy (MS) analysis. FTIR spectroscopic analysis revealed the presence of a carbonyl, amine, hydroxyl, and methyl as functional groups. The GC–MS analysis showed the presence of benzene dicarboxylic acid diethyl ester and pthalic acid as fatty acids while MALDI TOF MS/MS analysis shows lysin‐glycin as a hydrophilic dipeptide moiety. This study also demonstrates Microbial Enhanced Oil Recovery (MEOR) potential of the biosurfactant as more efficient than commercial ones. The biosurfactant obtained from waste orange peel as carbon source was able to facilitate a 20% higher recovery of diesel from sand pack recovery column.

中文翻译：

---

尼泊尔葡萄球菌（KY024500）生物表面活性剂对微生物强化采油的探索

长期以来，油脂化学一直被用作生物润滑剂，生物聚合物和生物表面活性剂。石油基产品的有效替代品。本研究探索了从地震多发地区岩石中分离出的一种新型菌株的生物表面活性剂潜力。根据形态，生化和16S rRNA测序分析，分离株被鉴定为尼泊尔葡萄球菌（KY024500）。生物表面活性剂的产量为2.39、1.39和0.9 g L ^-1分别使用甘油，废旧橙皮和柴油作为唯一碳源获得碳。基于通过砂包塔的采油实验结果，将废旧橙皮作为唯一碳源获得的生物表面活性剂进行了进一步分析。因此，将从废旧橙皮获得的生物表面活性剂进行溶剂萃取并通过柱色谱法纯化。由此获得的纯化的生物表面活性剂的特征在于傅立叶变换红外（FTIR），核磁共振（NMR），气相色谱-质谱（GC-MS）和MALDI TOF MS /质谱（MS）分析。FTIR光谱分析表明存在羰基，胺，羟基和甲基作为官能团。GC-MS分析表明存在苯二甲酸二乙酯和邻苯二甲酸作为脂肪酸，而MALDI TOF MS / MS分析表明溶血素-甘氨酸为亲水性二肽部分。这项研究还表明，该生物表面活性剂的微生物增强油回收（MEOR）潜力比商业表面活性剂更有效。从废橘皮作为碳源获得的生物表面活性剂能够促进从沙包回收塔中回收20％的柴油。