ABSTRACT

Egyptian garlic (Allium sativum) cultivars Balady and Sids 40 were exposed to different gamma radiation doses 10, 20, 30 and 40 Gy. Gamma radiation sensitivity of the two varieties was varied whereas cv. Sids 40 tolerated up to 30 Gy, whilst cv. Balady tolerated up to 10 Gy. Genomic DNA was isolated from irradiated and un-irradiated garlic leaves from both cultivars for SCoT markers. Organosulfur compound analysis by GC–MS was from irradiated and un-irradiated garlic cloves from M₁V₁ in both cultivars. Plant height and garlic bulbs size produced were reduced by increasing gamma radiation dose in M₁V₁. One clove bulbs mutations in cv. Sids 40 were reversed in the M₂V₂. Fifteen SCoT primers were used to evaluate the genetic diversity between garlic cultivars and among their treatments. These primers produced total amplified band 104 which polymorphic 43 (41.35%) with an average 6.93 band per primer, and 99 which polymorphic 31 (31.31%) with an average 6.6 band per primer in cvs. Balady and Sids 40, respectively. Polymorphic information content (PIC) values were varied from 0.12 (SCoT-36) to 0.46 (SCoT-28) with an average 0.29 (PIC < 0.5), and from zero (SCoT-16 and SCoT-22) to 0.46 (SCoT-12) with an average 3.63 (PIC < 0.5) in cvs. Balady and Sids 40, respectively. The effect of gamma radiation on organosulfur compounds as analyzed by GC–MS was limited to increased production as Diallyl disulfide; reduced production as 1,8-Dioxaspiro[4.5]decan-2-one4-(2-aminothiazol-4-yl)-7,7-dimethyl-; disappearance of some compounds as 2-Amino-4,4,6,6-tetramethyl-4,6-dihydro-thieno[2,3-c]furan-3-carbonitrile, or formation of some new compounds as Methanone,[4-][4-mthyl-5-(phenylmethyl)-4H-1,2,4-triazol-3-yl]thio]-3-nitrophenyl]2-pyridinyl-, Benzylsulfanylacetic acid, (benzothiazol-2-yl)methyl ester, and 2-Amino-4,4,6,6-tetramethyl-4,6-dihydro-thieno[2,3-c]furan-3-carbonitrile.

摘要

将埃及大蒜（Allium sativum）品种Balady和Sids 40暴露于不同的伽马辐射剂量10、20、30和40 Gy。两个品种的伽玛射线敏感性不同，而cv。Sid 40最高可承受30 Gy，而Cv。Balady容忍高达10 Gy。从两个品种的SCoT标记的辐照和未辐照大蒜叶中分离基因组DNA。GC-MS分析的有机硫化合物来自两个品种中M ₁ V _1的辐照和未辐照的蒜瓣。通过增加M ₁ V _1中的γ辐射剂量，可以降低植株高度和大蒜鳞茎的大小。一丁香球茎变异在简历。Sid 40在M ₂ V ₂中反转。使用15个SCoT引物评估大蒜品种之间及其处理之间的遗传多样性。这些引物在cvs中产生总扩增的条带104，其具有多态性43（41.35％），每个引物平均6.93条带；和99，其多态性31（31.31％），每个引物平均cvs条带。Balady和Sids 40。多态信息含量（PIC）值从0.12（SCoT-36）到0.46（SCoT-28），平均为0.29（PIC <0.5），从零（SCoT-16和SCoT-22）到0.46（SCoT- 12），平均cvs为3.63（PIC <0.5）。Balady和Sids 40。GC-MS分析表明，γ射线对有机硫化合物的影响仅限于增加二烯丙基二硫化物的产量；降低产量为1,8-二氧杂螺[4.5]癸-2--2-4-（2-氨基噻唑-4-基）-7,7-二甲基-；