ABSTRACT

The antibacterial effect of green tea and pomegranate peel extracts on Streptococcus mutans of orthodontically treated patients and its adherence to the tooth surface were investigated. Streptococcus mutans was isolated from saliva and plaque samples collected from orthodontic treated patients before the start of treatment and 3 months after the treatment. Gamma irradiated (5, and 10 kGy) and un-irradiated extracts of green tea leaves and pomegranate peels were prepared using polar (water, and 70% ethanol) and non-polar (hexane) solvents. The extraction yields, total phenol contents (TPC), and antibacterial activity of all the extracts were investigated. The irradiated (10 kGy) ethanol extracts of both pomegranate peels and green tea contained the maximum amount of TPC and showed superior antibacterial activity (maximum inhibition zone 28, and 35mm) and lowest minimum inhibitory concentration (25.0, and 12.50 mg/ml) against S. mutans, respectively when compared with the other extracts. The effect of various combinations of the irradiated (10 kGy) ethanol extracts of pomegranate peels and green tea on the adherence of S. mutans from orthodontic patients was finally evaluated.

摘要

研究了绿茶和石榴皮提取物对正畸治疗患者变形链球菌的抗菌作用及其对牙齿表面的粘附性。在治疗开始前和治疗后3个月，从口腔正畸治疗患者的唾液和斑块样本中分离出变形链球菌。使用极性（水和70％乙醇）和非极性（己烷）溶剂制备经γ辐射（5 kGy和10 kGy）和未辐射的绿茶叶和石榴皮提取物。研究了所有提取物的提取率，总酚含量（TPC）和抗菌活性。石榴皮和绿茶的经辐照（10 kGy）乙醇提取物均含有最大量的TPC，并显示出优异的抗菌活性（最大抑制区28，与其他提取物相比，分别对变形链球菌的最小抑菌浓度（3mm和35mm）和最低最低抑菌浓度（25.0和12.50 mg / ml）。最终评估了辐照的（10 kGy）石榴皮和绿茶乙醇提取物的各种组合对正畸患者变形链球菌粘附的影响。