ABSTRACT

The aim of the current study is to contribute to the basic understanding of Piriformosporaindica–Brassica campestris sp. chinensis interactions and to assess its impact on clubroot. This study revealed that P. indica, plant-root-colonizing fungus of Basidiomycetes, is capable to reduce the formation of clubroot in B. campestris sp. chinensis which is extremely susceptible to the root pathogen Plasmodiophora brassicae. Roots of the P. brassicae-infected plant were colonized with P. indica, as demonstrated by staining technique and microscopy. Like that, enhanced biomass was observed in P. indica co-inoculated plants by comparing with plants grown without P. indica as well as the plants infected with the pathogen only. Co-inoculation of the plant with both P. indica and P. brassicae reduced gall formation up to 61.60% by comparing plants infected with only P. brassicae as shown by the disease index. Higher level of flavonoids was also observed in P. indica inoculated plants which contribute to enhancing the plant immune system. Thus, a hypothesis was proposed that P. brassicae development in B. campestris sp. chinensis plants could be delayed with the inoculation of P. indica. Expression of P. brassicae genes which were differentially expressed during disease development was monitored using quantitative polymerase chain reaction and was corroborated with delayed pathogen development. The results suggest that P. indica and perhaps other beneficial endosymbionts could be an effective tool for the management of clubroot infection.

摘要

当前研究的目的是促进基本了解Piriformospora籼-油菜属。相互作用，并评估其对根茎的影响。这项研究表明，印度芽孢杆菌（Basidiomycetes）的植物根定殖真菌能够减少油菜芽孢杆菌（B. campestris sp。）的根茎形成。极易受根病原体芸苔属的中华。如通过染色技术和显微镜证实的那样，将感染毕赤酵母的植物的根部定植于印度假单胞菌。像这样，在印度假单胞菌中观察到生物量的增加通过与未接种印度假单胞菌的植物以及仅感染病原体的植物进行比较，共同接种了植物。通过比较仅感染了芸苔假单胞菌的植物，如疾病指数所示，将植物与印度。假单胞菌和芸苔假单胞菌共同接种可将胆汁形成减少多达61.60％。在接种印度假单胞菌的植物中还观察到较高的类黄酮含量，这有助于增强植物的免疫系统。因此，提出了一种假设，即在野油菜芽孢杆菌中的芸苔假单胞菌发育。接种中华d可能会延误中华绒螯蟹的发生; 芸苔假单胞菌的表达使用定量聚合酶链反应监测在疾病发展过程中差异表达的基因，并证实了延迟的病原体发展。结果表明，印度假单胞菌和其他有益的共生菌可能是治疗根部感染的有效工具。