ABSTRACT In this study, murine colon carcinoma (CT26) as well as non-cancerous Vero cells were exposed to various concentrations of essential oil (EO) obtained from the gum of F. persica (0.125 × 10−9 to 20 µl/ml and 0.125 × 10−9 to 80 µl/ml, respectively) for 24 h. The cytotoxicity and apoptosis were determined using MTT assay and acridine orange/ethidium bromide (AO/EB) staining, respectively. Clonogenic assay was also performed. Among 28 compounds that were determined by GC/MS, the major constituent of the EO was α-pinene (27.1%). A dose-dependent cytotoxicity was observed on CT26 cells with IC50 value of 0.0010 µl/ml. The cytotoxicity on Vero cells was relatively low with IC50 value of 0.3247µl/ml. The EO induced apoptosis in both cells and colony formation was also inhibited. In conclusion, the EO obtained from the gum of F. persica has cytotoxic and apoptotic effects on CT26 cell line with lesser effects on Vero cells.

中文翻译：

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阿魏胶精油对鼠结肠癌 (CT26) 和 Vero 细胞系的细胞毒活性

摘要 在这项研究中，鼠结肠癌 (CT26) 以及非癌性 Vero 细胞暴露于从 F. persica 树胶中获得的各种浓度的精油 (EO)（0.125 × 10-9 至 20 µl/ml 和0.125 × 10−9 至 80 µl/ml，分别）24 小时。分别使用 MTT 法和吖啶橙/溴化乙锭 (AO/EB) 染色确定细胞毒性和细胞凋亡。还进行了克隆形成试验。在 GC/MS 测定的 28 种化合物中，EO 的主要成分是 α-蒎烯 (27.1%)。在 CT26 细胞上观察到剂量依赖性细胞毒性，IC50 值为 0.0010 µl/ml。对Vero细胞的细胞毒性相对较低，IC50值为0.3247μl/ml。EO诱导的细胞凋亡和集落形成也受到抑制。总之，从F的树胶中获得的EO。