ABSTRACT Soy and maize are the most cultivated genetically modified (GM) crops in the world, which are commonly used in many food products. Establishing regulations for food products containing transgenic materials are obligatory in many countries in order to provide consumers with information. For this purpose, an accurate qualitative polymerase chain reaction (PCR) method was applied to investigate GM food products from Iran. In present study, a total of 90 non-labeled soy and maize samples were collected from Tehran’s market and their DNA were extracted by a kit. All samples were analyzed to screen the 35 S promoter and (nopaline synthase) NOS terminator elements. Having screened the GM positive samples, they were subjected to identification of specific transgenic events RR soy, Bt11 and MON810 maize with PCR. According to our results, 95% of soy and 60% of maize analyzed samples were positive for the 35 S promoter and NOS terminator. Furthermore, event-specific analysis indicated the presence of RR soy, Bt11 and MON810 maize in tested samples, while none of these food-derived samples demonstrated any GM label. The obtained results revealed that the necessity of monitoring system to provide a good reliable control of GM materials in food products and subsequently on their labeling.

中文翻译：

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基于 PCR 的定性检测伊朗转基因大豆和玉米产品

摘要 大豆和玉米是世界上种植最多的转基因 (GM) 作物，常用于许多食品中。为了向消费者提供信息，许多国家都必须对含有转基因材料的食品制定法规。为此，采用准确的定性聚合酶链反应 (PCR) 方法来研究来自伊朗的转基因食品。在本研究中，从德黑兰市场收集了总共 90 个未标记的大豆和玉米样品，并通过试剂盒提取了它们的 DNA。分析所有样品以筛选 35 S 启动子和（胭脂碱合酶）NOS 终止子元件。在筛选了 GM 阳性样品后，他们用 PCR 鉴定了特定的转基因事件 RR 大豆、Bt11 和 MON810 玉米。根据我们的结果，95% 的大豆和 60% 的玉米分析样品对 35 S 启动子和 NOS 终止子呈阳性。此外，特定事件的分析表明测试样品中存在 RR 大豆、Bt11 和 MON810 玉米，而这些食品来源的样品均未显示任何转基因标签。获得的结果表明，监测系统有必要对食品中的转基因材料以及随后的标签提供良好可靠的控制。