Low estrogen levels may predispose women to increased bodyweight and dyslipidemia. Previous studies from our laboratory suggest an involvement of depressed heat shock response (HSR) in this scenario because estrogen potently stimulates HSR. As heat treatment induces the expression of the anti-inflammatory heat shock proteins of the 70-kDa family (HSP70) and its accompanying HSR, we aimed to investigate whether chronic heat treatment promotes beneficial effects on biometric, lipid profile, oxidative stress, and HSR in ovariectomized rats. Wistar adult female rats (n = 32) were divided into four groups: control (C, n = 7), ovariectomized (OVX, n = 9), heat-treated (HT, n = 9), and heat-treated ovariectomized rats (OVX+HT, n = 7). HT and OVX+HT rats were anesthetized and submitted to heat treatment (once a week for 12 weeks) in a water bath (41 °C) to increase rats’ rectal temperature up to 41 °C for 15 min, while C and OVX animals were submitted to a 36 °C water bath. HT attenuated the weight gain induced by OVX and increased HDL cholesterol and triglyceride serum levels. Also, OVX rats showed increased total cholesterol and LDL cholesterol levels that were not influenced by HT. Interestingly, it was found that an overall trend for HT to decrease tissue catalase and superoxide dismutase antioxidant activities was paralleled by a decrease in malondialdehyde levels (indicative of lower lipoperoxidation), especially in the skeletal muscle. Surprisingly, OVX was not able to depress intracellular HSP70 expression in the skeletal muscle, as expected, and this remained unchanged with HT. However, chronic HT did enhance intracellular HSP70 contents in white adipose tissue of OVX animals. As both glucose and insulin tolerance tests were not affected by OVX, which was not modified by HT, we suppose that estrogen absence alone is not sufficient to determine a state of insulin resistance associated with low intramuscular HSP70 content.

中文翻译：

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长期热处理积极影响去卵巢大鼠的代谢状况：与热休克反应途径相关。

雌激素水平低可能使妇女容易增加体重和血脂异常。我们实验室先前的研究表明，在这种情况下，抑郁症涉及热休克反应（HSR），因为雌激素会有效刺激HSR。由于热处理诱导70-kDa家族的抗炎性热休克蛋白（HSP70）及其伴随的HSR的表达，我们旨在研究慢性热处理是否对生物测定，脂质分布，氧化应激和HSR产生有益的影响在去卵巢大鼠中。Wistar成年雌性大鼠（n  = 32）分为四组：对照组（C，n  = 7），去卵巢（OVX，n  = 9），经热处理（HT，n  = 9）和经热处理的去卵巢大鼠（OVX + HT，ñ = 7）。将HT和OVX + HT大鼠麻醉并在水浴（41°C）中进行热处理（每周一次，连续12周），以将大鼠的直肠温度升高至41°C持续15分钟，而C和OVX动物置于36°C水浴中。HT减弱了OVX引起的体重增加，并增加了HDL胆固醇和甘油三酸酯血清水平。另外，OVX大鼠的总胆固醇和LDL胆固醇水平升高，不受HT影响。有趣的是，发现HT降低组织过氧化氢酶和超氧化物歧化酶抗氧化活性的总体趋势与丙二醛水平的降低（指示较低的脂过氧化）平行，尤其是在骨骼肌中。出乎意料的是，OVX无法像预期的那样抑制骨骼肌中细胞内HSP70的表达，HT对此保持不变。但是，慢性HT确实增加了OVX动物白色脂肪组织中的细胞内HSP70含量。由于葡萄糖和胰岛素耐受性测试均不受OVX的影响，而OVX并未受到HT的影响，因此我们认为，单独的雌激素缺乏不足以确定与低肌内HSP70含量相关的胰岛素抵抗状态。