The present study was attempted to investigate the dynamics of HSPA1A and redox status in the spermatozoa and fluid of different segments of buck epididymis. Testes were collected from sexually mature and healthy bucks aged between 2 and 3 years. The fluid and spermatozoa from different segments (caput, corpus and cauda) were harvested for further processing and analysis. The concentration of HSPA1A in spermatozoa lysate and epididymal fluid and its relative mRNA expression in spermatozoa from different segments of epididymis were studied. The HSPA1A concentration in epididymal fluid was significantly (P < 0.01) higher in the corpus as compared with caput and cauda, whereas, its concentration and relative mRNA expression decreased significantly (P < 0.01) in the spermatozoa from caput to cauda. The activities of SOD, GR, GST, and concentrations of manoldialdehyde and ROS decreased significantly (P < 0.01) in the spermatozoa from caput to cauda. The glutathione concentration and GPx activity decreased significantly (P < 0.01) in the spermatozoa of cauda as compared with the corpus. The SOD activity and ROS concentration were significantly (P < 0.01) higher in corpus, and GR and GST activity were significantly (P < 0.01) higher in caput fluid as compared with corpus and cauda. It may be concluded that HSPA1A concentration and its relative mRNA expression in spermatozoa decreased progressively, and redox status was altered during transit from caput to cauda.

中文翻译：

---

山羊附睾不同部位精液和体液中HSPA1A和氧化还原状态的动态。

本研究试图调查降压附睾不同节段精子和体液中HSPA1A和氧化还原状态的动态。从两到三岁的性成熟和健康的雄鹿中收集睾丸。收集不同部位（（，ut体和马尾）的液体和精子，以进行进一步处理和分析。研究了附睾不同部位精子裂解液和附睾液中HSPA1A的浓度及其相对mRNA表达。与卡普达 和马尾相比，epi体附睾液中HSPA1A的浓度显着高（P <0.01），而其浓度和相对mRNA表达则显着降低（P <0.01）在从帽角到马尾的精子中。从帽角 到马尾精子中，SOD，GR，GST的活性以及manoldialdehyde和ROS的浓度均显着降低（P <0.01）。 与主体相比，马尾精子中的谷胱甘肽浓度和GPx活性显着降低（P <0.01）。 与语料库和马尾语相比， 在语料库中SOD活性和ROS浓度显着高（P <0.01），并且在液汁中GR和GST活性显着（P <0.01）。可以得出结论，在从帽角到马尾的过程中，精子中HSPA1A的浓度及其相对mRNA表达逐渐降低，并且氧化还原状态发生了改变。