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Metagenomic characterization of bacterial biofilm in four food processing plants in Colombia
Brazilian Journal of Microbiology ( IF 2.2 ) Pub Date : 2020-03-27 , DOI: 10.1007/s42770-020-00260-x
Arley Caraballo Guzmán 1 , Maria Isabel González Hurtado 2 , Yesid Cuesta-Astroz 1 , Giovanny Torres 1
Affiliation  

Bacteria inside biofilms are more persistent and resistant to stress conditions found in the production environment of food processing plants, thus representing a constant risk for product safety and quality. Therefore, the aim of this study was to characterize, using 16S rRNA sequencing, the bacterial communities from biofilms found in four food processing plants (P1, P2, P3, and P4). In total, 50 samples from these four processing plants were taken after cleaning and disinfection processes. Four phyla: Proteobacteria, Firmicutes, Actinobacteria, and Bacteroides represented over 94% of the operational taxonomic units found across these four plants. A total of 102 families and 189 genera were identified. Two genera, Pseudomonas spp. and Acinetobacter spp., were the most frequently found (93.47%) across the four plants. In P1, Pseudomonas spp. and Lactobacillus spp. were the dominant genera, whereas Lactobacillus spp. and Streptococcus spp. were identified in P2. On the other hand, biofilms found in P3 and P4 mainly consisted of Pseudomonas spp. and Acinetobacter spp. Our results indicate that different bacterial genera of interest to the food industry due to their ability to form biofilm and affect food quality can coexist inside biofilms, and as such, persist in production environments, representing a constant risk for manufactured foods. In addition, the core microbiota identified across processing plants evaluated was probably influenced by type of food produced and cleaning and disinfection processes performed in each one of these.

中文翻译:

哥伦比亚四家食品加工厂细菌生物膜的宏基因组特征

生物膜内的细菌更持久,对食品加工厂生产环境中的压力条件具有抵抗力,因此对产品安全和质量构成持续的风险。因此,本研究的目的是使用 16S rRNA 测序来表征来自四个食品加工厂(P1、P2、P3 和 P4)中发现的生物膜的细菌群落。在清洁和消毒过程之后,总共从这四个加工厂采集了 50 个样本。四个门:变形菌门、厚壁菌门、放线菌门和拟杆菌门占这四种植物中发现的可操作分类单元的 94% 以上。共鉴定出102科189属。两个属,假单胞菌属。和不动杆菌属,是四种植物中最常见的 (93.47%)。在 P1 中,假单胞菌属。和乳酸菌属。是优势属,而乳酸杆菌属。和链球菌属。在 P2 中被识别。另一方面,在 P3 和 P4 中发现的生物膜主要由假单胞菌属。和不动杆菌属。我们的研究结果表明,食品工业感兴趣的不同细菌属,由于它们能够形成生物膜并影响食品质量,可以在生物膜内共存,因此,在生产环境中持续存在,代表制造食品的持续风险。此外,在评估的加工厂中确定的核心微生物群可能受到生产的食品类型以及在每个加工厂中执行的清洁和消毒过程的影响。在 P3 和 P4 中发现的生物膜主要由假单胞菌属组成。和不动杆菌属。我们的研究结果表明,食品工业感兴趣的不同细菌属,由于它们能够形成生物膜并影响食品质量,可以在生物膜内共存,因此,在生产环境中持续存在,对加工食品构成持续风险。此外,在评估的加工厂中确定的核心微生物群可能受到生产的食品类型以及在每个加工厂中执行的清洁和消毒过程的影响。在 P3 和 P4 中发现的生物膜主要由假单胞菌属。和不动杆菌属。我们的研究结果表明,食品工业感兴趣的不同细菌属,由于它们能够形成生物膜并影响食品质量,可以在生物膜内共存,因此,在生产环境中持续存在,代表制造食品的持续风险。此外,在评估的加工厂中确定的核心微生物群可能受到生产的食品类型以及在每个加工厂中执行的清洁和消毒过程的影响。在生产环境中持续存在,这对制造的食品来说意味着持续的风险。此外,在评估的加工厂中确定的核心微生物群可能受到生产的食品类型以及在每个加工厂中执行的清洁和消毒过程的影响。在生产环境中持续存在,这对制造的食品来说意味着持续的风险。此外,在评估的加工厂中确定的核心微生物群可能受到生产的食品类型以及在每个加工厂中执行的清洁和消毒过程的影响。
更新日期:2020-03-27
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