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Multidrug-resistant Acinetobacter baumannii: differential adherence to HEp-2 and A-549 cells
Brazilian Journal of Microbiology ( IF 2.2 ) Pub Date : 2020-03-16 , DOI: 10.1007/s42770-020-00252-x
Gabrielle Limeira Genteluci 1, 2 , Daniela Betzler Cardoso Gomes 1, 2 , Daniella Pereira 1 , Marta de Campos Neves 1 , Maria José de Souza 3 , Karyne Rangel 1 , Maria Helena Simões Villas Bôas 1
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Acinetobacter baumannii has been associated with antimicrobial resistance and ability to form biofilms. Furthermore, its adherence to host cells is an important factor to the colonization process. Therefore, this study intended to identify some virulence factors that can explain the success of A. baumannii in causing nosocomial infections. We studied 92 A. baumannii isolates collected from hospitals in Rio de Janeiro, Brazil. Isolates were identified and the susceptibility to antimicrobials was determined. Oxacilinase type β-lactamase encoding genes were amplified by polymerase chain reaction, and genetic diversity was investigated by pulsed-field gel electrophoresis (PFGE). In addition, biofilm formation on polystyrene plates using crystal violet staining was quantified, and adherence to human cell lines was evaluated. Eighty-six isolates were multidrug-resistant, of which 93% were carbapenem-resistant. All isolates had the blaOXA-51 gene and 94% had the blaOXA-23 gene, other searched blaOXA genes were not detected. PFGE typing showed two predominant clones, and biofilm production was observed in 79% of isolates. A. baumannii isolates adhered better to HEp-2 cell compared with A-549 cell. Clones A, B, E, and F showed a significantly increased adherence to HEp-2 compared with adherence to A-549 cell. Our findings revealed that A. baumannii isolates had high frequencies of resistance to antimicrobial agents, ability to form biofilm, and capacity to adhere to HEp-2 cells.

中文翻译:

多重耐药鲍曼不动杆菌:对 HEp-2 和 A-549 细胞的不同粘附

鲍曼不动杆菌与抗菌素耐药性和形成生物膜的能力有关。此外,它对宿主细胞的粘附是定植过程的一个重要因素。因此,本研究旨在确定一些可以解释鲍曼不动杆菌成功引起医院感染的毒力因素。我们研究了从巴西里约热内卢的医院收集的 92 株鲍曼不动杆菌。鉴定了分离物并确定了对抗微生物剂的敏感性。通过聚合酶链反应扩增苯唑西林酶型β-内酰胺酶编码基因,并通过脉冲场凝胶电泳(PFGE)研究遗传多样性。此外,使用结晶紫染色对聚苯乙烯板上的生物膜形成进行量化,并评估对人类细胞系的粘附。86 株分离株具有多重耐药性,其中 93% 对碳青霉烯类耐药。所有分离株均含有blaOXA-51基因,94%的分离株含有blaOXA-23基因,未检测到其他检索到的blaOXA基因。PFGE 分型显示了两个主要克隆,并且在 79% 的分离株中观察到了生物膜产生。与 A-549 细胞相比,鲍曼不动杆菌分离株对 HEp-2 细胞的粘附更好。与对 A-549 细胞的粘附相比,克隆 A、B、E 和 F 对 HEp-2 的粘附显着增加。我们的研究结果表明,鲍曼不动杆菌分离株对抗菌剂具有很高的耐药性、形成生物膜的能力以及粘附于 HEp-2 细胞的能力。PFGE 分型显示了两个主要克隆,并且在 79% 的分离株中观察到了生物膜产生。与 A-549 细胞相比,鲍曼不动杆菌分离株对 HEp-2 细胞的粘附更好。与对 A-549 细胞的粘附相比,克隆 A、B、E 和 F 对 HEp-2 的粘附显着增加。我们的研究结果表明,鲍曼不动杆菌分离株对抗菌剂具有很高的耐药性、形成生物膜的能力以及粘附于 HEp-2 细胞的能力。PFGE 分型显示了两个主要克隆,并且在 79% 的分离株中观察到了生物膜产生。与 A-549 细胞相比,鲍曼不动杆菌分离株对 HEp-2 细胞的粘附更好。与对 A-549 细胞的粘附相比,克隆 A、B、E 和 F 对 HEp-2 的粘附显着增加。我们的研究结果表明,鲍曼不动杆菌分离株对抗菌剂具有很高的耐药性、形成生物膜的能力以及粘附于 HEp-2 细胞的能力。
更新日期:2020-03-16
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