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Sutterella faecalis sp. nov., isolated from human faeces
Journal of Microbiology ( IF 3 ) Pub Date : 2020-01-29 , DOI: 10.1007/s12275-020-9396-9
Byeong Seob Oh , Ji-Sun Kim , Seung Yeob Yu , Seoung Woo Ryu , Seung-Hwan Park , Se Won Kang , Jam-Eon Park , Seung-Hyeon Choi , Kook-Il Han , Keun Chul Lee , Mi Kyung Eom , Min Kuk Suh , Han Sol Kim , Dong Ho Lee , Hyuk Yoon , Byung-Yong Kim , Je Hee Lee , Jung-Sook Lee , Ju Huck Lee

An obligately anaerobic, Gram-stain-negative, non-motile, non-spore-forming, and coccobacilli-shaped bacterial strain, designated KGMB03119T, was isolated from human faeces from a Korean. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the isolate was a member of the genus Sutterella and most closely related to Sutterlla wadsworthensis KCTC 15691T (96.8% 16S rRNA gene sequence similarity). The DNA G + C content of strain KGMB03119T was 58.3 mol% as determined from its whole genome sequence. Strain KGMB03119T was asaccharolytic, catalase-positive, oxidase- and urease-negative. Furthermore, the isolate was positive for alkaline phosphatase, leucine arylamidase, acid phosphatase, arginine arylamidase, alanine arylamidase, and glycine arylamidase. The major cellular fatty acids (> 10%) of the isolate were C18:1ω9c and C16:0. Methylmenaquinone-5 (MMK-5, 100%) was the predominant isoprenoid quinone in the isolate. Based on the phylogenetic, physiological, and chemotaxonomic characteristics, strain KGMB03119T represents a novel species, for which the name Sutterella faecalis sp. nov. is proposed. The type strain is KGMB03119T (= KCTC 15823T = NBRC 114254T).

中文翻译:

粪沙门氏菌 十一月,与人类粪便隔离

一个专性厌氧革兰氏染色阴性的,不动的,非孢子形成,和球杆菌形细菌菌株,指定KGMB03119 Ť,从人粪便中分离从一个朝鲜语。基于16S rRNA基因序列的系统进化分析表明,该分离物是Sutterella属的成员,与Sutterlla wadsworthensis KCTC 15691 T的亲缘关系最密切(96.8%的16S rRNA基因序列相似性)。应变KGMB03119的DNA G + C含量Ť率为58.3%(摩尔),从其全基因组序列测定。菌株KGMB03119 T是糖酵解酶,过氧化氢酶阳性,氧化酶和脲酶阴性。此外,分离物对碱性磷酸酶,亮氨酸芳酰胺酶,酸性磷酸酶,精氨酸芳酰胺酶,丙氨酸芳酰胺酶和甘氨酸芳酰胺酶呈阳性。该分离物的主要细胞脂肪酸(> 10%)为C 18:1个ω 9C和C 16:0。甲基甲萘醌-5(MMK-5,100%)是分离物中的主要类异戊二烯醌。根据系统发育,生理和化学分类学特征,菌株KGMB03119 T代表一个新物种,其名称为Sutterella faecalis sp。十一月 被提议。类型应变为KGMB03119 T(= KCTC 15823 T = NBRC 114254 T)。
更新日期:2020-01-29
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