Branching is an important factor affecting the yield of Brassica napus L. Generally, however, field-grown B. napus cannot develop branches from the middle and lower parts of the plant, although axillary buds are present in these parts. To explore the cause of this phenomenon at the mRNA level, we analyzed buds from the top, upper, middle and lower parts of B. napus by RNA-SEq. In total, 27,992 expressed genes were found, 479 of which were considered to be closely related to axillary bud development. Genes related to the regulation of sugar signaling and plant hormone signal transduction were subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. Seven types of transcription factors, including phytochrome-interacting factors (PIFs), homeobox 1 (HB-1), light sensitive hypocotyls 4 (LSH4), BRANCHED 1 (BRC1), WRKY DNA-binding proteins (WRKYs) and Transducin/WD40 repeat-like superfamily proteins (WD40s), were enriched. The soluble sugar content in the axillary buds was assessed and found to differ significantly among the four regions of the plant; in short, the soluble sugar content decreased from top to bottom. In this study, we provide a transcriptional profile of axillary buds from different parts of B. napus plants; these data will be important for future studies on the growth and development of axillary buds.

中文翻译：

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在转录组水平上通过糖和激素信号调节甘蓝型油菜腋芽的生长

分枝是影响甘蓝型油菜产量的重要因素。但一般来说，田间种植的油菜不能从植株的中下部发育分枝，尽管这些部分存在腋芽。为了在 mRNA 水平探索这种现象的原因，我们通过 RNA-SEq 分析了欧洲油菜顶部、上部、中部和下部的芽。共发现27,992个表达基因，其中479个被认为与腋芽发育密切相关。与糖信号和植物激素信号转导相关的基因进行了基因本体论 (GO) 和京都基因和基因组百科全书 (KEGG) 富集分析。七种转录因子，包括光敏色素相互作用因子（PIFs）、同源框1（HB-1）、光敏下胚轴4（LSH4）、BRANCHED 1 (BRC1)、WRKY DNA 结合蛋白 (WRKYs) 和 Transducin/WD40 重复样超家族蛋白 (WD40s) 得到了丰富。评估了腋芽中的可溶性糖含量，发现植物的四个区域之间存在显着差异；总之，可溶性糖含量自上而下递减。在这项研究中，我们提供了欧洲油菜植物不同部位腋芽的转录谱；这些数据对于未来关于腋芽生长和发育的研究非常重要。在这项研究中，我们提供了欧洲油菜植物不同部位腋芽的转录谱；这些数据对于未来关于腋芽生长和发育的研究非常重要。在这项研究中，我们提供了欧洲油菜植物不同部位腋芽的转录谱；这些数据对于未来关于腋芽生长和发育的研究非常重要。