Although the presence of scent was described for several species of Rhamnaceae, localization, morphology and structure of osmophores were unknown. We studied different species of the tribes Rhamneae (Rhamnoids clade), Pomaderreae, Colletieae, Paliureae (Ziziphoids clade) and the species Alphitonia excelsa (unknown tribe, Ziziphoids clade) . We expect to have a better comprehension of these structures and provide information on which morphological and anatomical characters may support the phylogeny of the family. We localized the osmophores in the margins and top of the sepals using neutral red. Histochemical tests were made on transverse hand-cut sections of fresh sepals. Observations were made with stereoscopic and bright field microscopes, scanning and transmission electron microscopes. Papillae were observed in the zones with positive reaction to reagents. Different kinds of hairs are present in the sepal epidermis besides papillae. Epidermal cells present a striate cuticle with canals and cavities. Druses are abundant in most species. The ultrastructure of epidermal and subepidermal cells shows high metabolic activity: there are vesicles, mitochondria, endoplasmic reticulum, dictyosomes, plastids with lipids and starch. The vascularization is well developed and reaches the top of the sepal where the principal area of volatile components production is localized. The location and abundance of papillae are the most important traits that allow us recognize and characterize the osmophores in Rhamnaceae. There are no clear anatomical and morphological features exclusive of one clade or tribe. Therefore, in contrast to other sporophytic structures of this family, osmophores do not seem to have any systematic value.

中文翻译：

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鼠李科物种渗透压的定位、形态、解剖和超微结构

尽管在几种鼠李科物种中描述了气味的存在，但渗透素的定位、形态和结构是未知的。我们研究了鼠李科（Rhamnoids clade）、Pomaderreae、Colletieae、Paliureae（Ziziphoids 进化枝）和 Alphitonia excelsa（未知部落，Ziziphoids 进化枝）的不同物种。我们希望对这些结构有更好的理解，并提供有关哪些形态学和解剖学特征可能支持该科的系统发育的信息。我们使用中性红色定位了萼片边缘和顶部的渗透素。对新鲜萼片的横向手工切割切片进行组织化学测试。用立体和明场显微镜、扫描和透射电子显微镜进行观察。在对试剂呈阳性反应的区域中观察到乳头状突起。除了乳头外，萼片表皮中还存在不同种类的毛发。表皮细胞呈现带管和腔的纹状角质层。在大多数物种中，果核都很丰富。表皮和表皮下细胞的超微结构显示出高代谢活性：有囊泡、线粒体、内质网、dictyosomes、带有脂质和淀粉的质体。血管发育良好并到达萼片顶部，在那里是挥发性成分产生的主要区域。乳头的位置和丰度是使我们能够识别和表征鼠李科渗透压的最重要特征。除了一个进化枝或部落外，没有明确的解剖学和形态学特征。所以，