For both bioprospecting of oleaginous microalgae and routine analysis of lipid levels in these microorganisms, reliable techniques are needed. Methods usually used for these purposes include fluorescent staining of lipids and gravimetric determination. In this research, we evaluate the application of a specific Oil Red O staining technique to detect accumulated lipids in the microalgae Chlorella sp., Nitzschia laevis, and Crypthecodinium cohnii. In addition, the viability to evaluate lipid accumulation levels in these microalgae is also analyzed. This methodology offers a less expensive alternative in the study of lipid accumulation levels in oleaginous microalgae since it requires small amounts of cells and low-complexity analytical equipment for its application.

对于含油微藻的生物勘探和这些微生物中脂质水平的常规分析，都需要可靠的技术。通常用于这些目的的方法包括脂质的荧光染色和重量分析法。在这项研究中，我们评估了一种特定的油红O染色技术在微藻藻，小球藻，Nitzschia laevis和Crypthecodinium cohnii中积累的脂质的检测应用。。此外，还分析了评估这些微藻中脂质积累水平的可行性。该方法为研究含油微藻中脂质的积累水平提供了一种较便宜的替代方法，因为它需要少量的细胞和低复杂度的分析设备才能用于其应用。