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In Silico Analysis of ACE Inhibitory Peptides from Chloroplast Proteins of Red Alga Grateloupia asiatica.
Marine Biotechnology ( IF 3 ) Pub Date : 2020-03-24 , DOI: 10.1007/s10126-020-09959-2
Kana Sumikawa 1 , Kentaro Takei 1 , Yuya Kumagai 2 , Takeshi Shimizu 3 , Hajime Yasui 4 , Hideki Kishimura 2
Affiliation  

Inhibition of angiotensin I–converting enzyme (ACE) is one of the key factors to repress high blood pressure. Although many studies have been reported that seaweed protein hydrolysates showed the ACE inhibitory activity, the comprehensive understanding of the relationship was still unclear. In this study, we employed chloroplast genome for in silico analysis and compared it with in vitro experiments. We first extracted water-soluble proteins (WSP) from red alga Grateloupia asiatica, which contained mainly PE, PC, APC, and Rbc, and prepared WSP hydrolysate by thermolysin, resulting that the hydrolysate showed ACE inhibitory activity. Then, we determined the complete chloroplast genome of G. asiatica (187,518 bp: 206 protein-coding genes, 29 tRNA, and 3 rRNA) and clarified the amino acid sequences of main WSP, i.e., phycobiliproteins and Rubisco, to perform in silico analysis. Consequently, 190 potential ACE inhibitory peptides existed in the main WSP sequences, and 21 peptides were obtained by in silico thermolysin digestion. By comparing in vitro and in silico analyses, in vitro ACE inhibitory activity was correlated to the IC50 value from in silico digestion. Therefore, in silico approach provides insight into the comprehensive understanding of the potential bioactive peptides from seaweed proteins.

中文翻译:

在计算机模拟中分析红藻海藻的叶绿体蛋白中的ACE抑制肽。

抑制血管紧张素I转换酶(ACE)是抑制高血压的关键因素之一。尽管已报道许多研究表明海藻蛋白水解物具有ACE抑制活性,但对这种关系的全面了解仍不清楚。在这项研究中,我们采用叶绿体基因组进行计算机分析,并将其与体外实验进行了比较。我们首先从主要包含PE,PC,APC和Rbc的红藻Grateloupia asiatica中提取了水溶性蛋白(WSP),并通过嗜热菌蛋白酶制备了WSP水解产物,结果该水解产物显示出ACE抑制活性。然后,我们确定了G的完整叶绿体基因组。Asiatica(187,518 bp:206个蛋白质编码基因,29个tRNA和3个rRNA),并阐明了主要WSP的氨基酸序列,即藻胆蛋白和Rubisco,以进行计算机分析。因此,在主要的WSP序列中存在190种潜在的ACE抑制肽,并且通过计算机热裂解酶消化获得了21种肽。通过比较体外和计算机分析,体外ACE抑制活性与计算机消解的IC 50值相关。因此,in silico方法可提供对海藻蛋白质潜在生物活性肽的全面了解的见识。
更新日期:2020-03-24
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