Human umbilical cord mesenchymal stem cells (hUC-MSCs) are a pivotal source of therapeutically active cells for regenerative medicine due to their multipotent differentiation potential, immunomodulatory and anti-inflammatory proprieties, as well as logistical collection advantages without ethical concerns. However, it remains poorly understood whether MSCs from different compartments of the human umbilical cord are therapeutically superior than others. In this study, MSCs were isolated from Wharton’s jelly (WJ-MSCs), perivascular region (PV-MSCs) and cord lining (CL-MSCs) of hUC. These cells expressed the mesenchymal markers (CD90, CD73), stemness marker (OCT4), endothelial cell adhesion molecular marker (CD146), and the monocyte/macrophage marker (CD14) found within the MSC population implicated as a key regulator of inflammatory responses to hypoxia, was displayed by WJ-, PV-, and CL-MSCs respectively. A direct consequence of oxygen and glucose deprivation during stroke and reperfusion is impaired mitochondrial function that contributes to cellular death. Emerging findings of mitochondria transfer provide the basis for the replenishment of healthy mitochondria as a strategy for the treatment of stroke. Cell Energy Phenotype and Mito Stress tests were performed the energy metabolic profile of the three MSC populations and their mitochondrial function in both ambient and OGD cell culture conditions. PV-MSCs showed the highest mitochondrial activity. CL-MSCs were the least affected by OGD/R condition, suggesting their robust survival in ischemic environment. In this study, MSC populations in UC possess comparable metabolic capacities and good survival under normal and hypoxic conditions suggesting their potential as transplantable cells for mitochondrial-based stem cell therapy in stroke and other ischemic diseases.

中文翻译：

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正常和病理条件下三种不同脐带间充质干细胞群体的能量代谢分析。

人脐带间充质干细胞（hUC-MSC）是再生医学治疗活性细胞的重要来源，因为它们具有多能分化潜能，免疫调节和抗炎特性，并且在物流方面具有优势，而无需担心伦理问题。但是，人们对来自人脐带不同隔室的MSCs是否在治疗上优于其他MSCs仍然知之甚少。在这项研究中，MSCs是从hUC的沃顿胶冻（WJ-MSCs），血管周围区域（PV-MSCs）和脐带衬里（CL-MSCs）中分离出来的。这些细胞表达间充质标记（CD90，CD73），干性标记（OCT4），内皮细胞粘附分子标记（CD146），WJ-，PV-和CL-MSC分别显示了在MSC群体中发现的单核细胞/巨噬细胞标志物（CD14），其被认为是对缺氧的炎症反应的关键调节剂。中风和再灌注过程中氧气和葡萄糖剥夺的直接后果是线粒体功能受损，导致细胞死亡。线粒体转移的新发现为补充健康的线粒体作为中风的治疗策略提供了基础。在环境和OGD细胞培养条件下，对三个MSC种群的能量代谢谱及其线粒体功能进行了细胞能量表型和线粒体应力测试。PV-MSC表现出最高的线粒体活性。CL-MSC受OGD / R条件的影响最小，提示它们在缺血环境中的生存能力强。在这项研究中，UC中的MSC人群具有相当的代谢能力，并且在正常和低氧条件下都具有良好的存活率，这表明它们具有作为线粒体干细胞治疗中风和其他缺血性疾病的可移植细胞的潜力。