Decellularized tissues and organs have aroused considerable interest for developing functional bio-scaffolds as natural templates in tissue engineering applications. More recently, the use of natural extracellular matrix (ECM) extracted from the in vitro cell cultures for cellular applications have come into question. It is well known that the microenvironment largely defines cellular properties. Thus, we have anticipated that the ECMs of the cells with different potency levels should likely possess different effects on cell cultures. To test this, we have comparatively evaluated the differentiative effects of ECMs derived from the cultures of human somatic dermal fibroblasts, human multipotent bone marrow mesenchymal stem cells, and human induced pluripotent stem cells on somatic dermal fibroblasts. Although challenges remain, the data suggest that the use of cell culture-based extracellular matrices perhaps may be considered as an alternative approach for the differentiation of even somatic cells into other cell types.

中文翻译：

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去细胞的细胞培养ECM充当细胞分化诱导剂。

去细胞的组织和器官引起了人们对于将功能性生物支架开发为组织工程应用中的天然模板的极大兴趣。最近，从体外细胞培养物中提取的天然细胞外基质（ECM）在细胞应用中的应用受到了质疑。众所周知，微环境在很大程度上定义了细胞特性。因此，我们预计具有不同效力水平的细胞的ECM可能对细胞培养物具有不同的影响。为了测试这一点，我们已经比较评估了源自人体真皮成纤维细胞，人多能骨髓间充质干细胞和人诱导多能干细胞培养物的ECM对体真皮成纤维细胞的分化作用。尽管挑战依然存在，