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The Trichoderma viride F-00612 consortium tolerates 2-amino-3 H -phenoxazin-3-one and degrades nitrated benzo[ d ]oxazol-2(3 H )-one
Chemoecology ( IF 1.8 ) Pub Date : 2020-01-24 , DOI: 10.1007/s00049-020-00300-w
Nataliya Voloshchuk , Vadim Schütz , Laura Laschke , Andrii P. Gryganskyi , Margot Schulz

Numerous allelopathic plant secondary metabolites impact plant–microorganism interactions by injuring plant-associated beneficial bacteria and fungi. Fungi belonging to the genus Trichoderma positively influence crops, including benzoxazinone-containing maize. However, benzoxazinones and their downstream metabolites such as benzoxazolinone and phenoxazinones are often fungitoxic. Specimen Trichoderma viride F-00612 was found to be insensitive to 100-µM phenoxazinone and 500-µM benzoxazolinone. Screening of 46 additional specimens of ascomycetes revealed insensitivity to phenoxazinones among fungi that cause disease in benzoxazinone-producing cereal crops, whereas many other ascomycetes were highly sensitive. In contrast, most of the screened fungi were insensitive to benzoxazolinone. T. viride F-00612 was associated with bacteria and, thus, existed as a consortium. By contrast, Enterobacter species and Acinetobacter calcoaceticus were prominent in the original specimen, and Bacillus species predominated after antibiotic application. Prolonged cultivation of T. viride F-00612 in liquid medium and on Czapek agar in the presence of < 100 µM phenoxazinone and < 500 µM benzoxazolinone resulted in a massive loss of bacteria accompanied by impacted fungal growth in the presence of phenoxazinone. The original consortium was actively involved in implementing metabolic sequences for the degradation and detoxification of nitrated benzoxazolinone derivatives. The 2-aminophenol was rapidly converted into acetamidophenol, but benzoxazolinone, methoxylated benzoxazolinone, and picolinic acid remained unchanged. Excluding phenoxazinone, none of the tested compounds markedly impaired fungal growth in liquid culture. In conclusion, members of the T. viride F-00612 consortium may contribute to the ability to manage benzoxazinone downstream products and facilitate BOA-6-OH degradation via nitration.

中文翻译:

绿色木霉F-00612联盟可耐受2-氨基-3 H-苯恶嗪-3-酮并降解硝化的苯并[d]恶唑-2(3 H)-酮

许多化感植物次生代谢产物会伤害植物相关的有益细菌和真菌,从而影响植物与微生物的相互作用。属于木霉属的真菌对包括含苯并恶嗪酮的玉米在内的农作物产生积极影响。但是,苯并恶嗪酮及其下游代谢产物(如苯并恶唑啉酮和苯恶嗪酮)通常具有真菌毒性。发现标本木霉木霉F-00612对100 µM苯恶嗪酮和500 µM苯并恶唑啉酮不敏感。对另外46个子囊菌标本的筛选显示,在引起苯并恶嗪酮生产的谷物作物中引起疾病​​的真菌中,对苯氧嗪酮不敏感,而许多其他子囊菌则高度敏感。相反,大多数筛选的真菌对苯并恶唑啉酮不敏感。T. viride F-00612与细菌有关,因此以财团的形式存在。相比之下,肠杆菌种和钙乙酸不动杆菌在原始标本中突出,而抗生素应用后以芽孢杆菌种为主。长期栽培的T. viride在<100 µM苯恶嗪酮和<500 µM苯并恶唑啉酮的存在下,液体培养基中和Czapek琼脂上的F-00612会导致大量细菌损失,并伴随着苯并恶嗪酮的存在而影响真菌的生长。最初的财团积极参与实施代谢序列,以实现硝化苯并恶唑啉酮衍生物的降解和解毒。2-氨基苯酚迅速转化为乙酰氨基苯酚,但苯并恶唑啉酮,甲氧基化苯并恶唑啉酮和吡啶甲酸保持不变。除苯恶嗪酮外,所有受试化合物均无明显损害液体培养中的真菌生长。最后,T。viride成员 F-00612财团可能有助于管理下游苯并恶嗪酮产品并通过硝化促进BOA-6-OH降解。
更新日期:2020-01-24
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