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Potential Beneficial Effects of Digitaria ciliaris Flower Absolute on the Wound Healing-Linked Activities of Fibroblasts and Keratinocytes
Planta Medica ( IF 2.7 ) Pub Date : 2020-02-11 , DOI: 10.1055/a-1101-9326
Soo Min Park 1 , Kyung Jong Won 2 , Dae Il Hwang 1, 3 , Do Yoon Kim 4 , Ha Bin Kim 1 , Yali Li 1 , Hwan Myung Lee 1
Affiliation  

Digitaria ciliaris is widely reported to be a problematic weed in agricultural areas and is mainly used as an indicator plant for the development of herbicides. However, its bioactivities on skin regeneration and wound healing have not been investigated. In the present study, we investigated the effects of D. ciliaris flower absolute on skin wound healing and skin regeneration-related events, that is, proliferation, migration, and collagen biosynthesis, in human fibroblasts and keratinocytes. For this study, we extracted absolute from the D. ciliaris flower by solvent extraction and identified the composition of D. ciliaris flower absolute using GC/MS analysis. We also tested the effect of D. ciliaris flower absolute in CCD986sk fibroblasts and/or HaCaT keratinocytes using the WST assay and 5-bromo-2'-deoxyuridine incorporation assay, Boyden chamber assay, ELISA, sprouting assay, and immunoblotting. GC/MS analysis of D. ciliaris flower absolute revealed that it contained 15 compounds. The absolute increased the proliferations of keratinocytes and fibroblasts and the migration of fibroblasts but did not affect cell viabilities. In addition, it enhanced the syntheses of type I and IV collagen in fibroblasts, but not in keratinocytes. The sprouting assay showed increased sprout outgrowth of fibroblasts. In addition, D. ciliaris flower absolute induced the phosphorylation of extracellular signal-regulated kinase 1/2 and p38 mitogen-activated protein kinase in fibroblasts. These results indicate that D. ciliaris flower absolute may promote skin wound healing/regeneration by inducing the proliferation, migration, and collagen synthesis of fibroblasts, as well as the proliferation of keratinocytes. Therefore, D. ciliaris flower absolute may be a potential natural source for cosmetic or pharmaceutical agents that promote skin wound healing/regeneration.

中文翻译:

Digitaria ciliaris Flower Absolute 对成纤维细胞和角质形成细胞伤口愈合相关活性的潜在有益影响

据报道,马尾草是农业地区的一种有问题的杂草,主要用作开发除草剂的指示植物。然而,尚未研究其对皮肤再生和伤口愈合的生物活性。在本研究中,我们研究了 D. ciliaris flower absolute 对人成纤维细胞和角质形成细胞中皮肤伤口愈合和皮肤再生相关事件(即增殖、迁移和胶原蛋白生物合成)的影响。在本研究中,我们通过溶剂萃取法从 D. ciliaris 花中提取了纯水,并使用 GC/MS 分析确定了 D. ciliaris 花纯水的组成。我们还使用 WST 测定和 5-溴-2'-脱氧尿苷掺入测定测试了 D. ciliaris 花绝对对 CCD986sk 成纤维细胞和/或 HaCaT 角质形成细胞的影响,博伊登室测定、ELISA、发芽测定和免疫印迹。D. ciliaris 花净油的 GC/MS 分析显示它含有 15 种化合物。绝对增加角质形成细胞和成纤维细胞的增殖和成纤维细胞的迁移,但不影响细胞活力。此外,它增强了成纤维细胞中 I 型和 IV 型胶原蛋白的合成,但不增强角质形成细胞中的合成。发芽测定显示成纤维细胞的发芽生长增加。此外,D. ciliaris flower absolute 诱导成纤维细胞中细胞外信号调节激酶 1/2 和 p38 丝裂原活化蛋白激酶的磷酸化。这些结果表明 D. ciliaris flower absolute 可通过诱导成纤维细胞的增殖、迁移和胶原合成来促进皮肤伤口愈合/再生,以及角质细胞的增殖。因此, D. ciliaris 花绝对可能是促进皮肤伤口愈合/再生的化妆品或药剂的潜在天然来源。
更新日期:2020-02-11
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