The endophytic microbiome in medicinal plants is rich and diverse, but few studies have followed the endophytic microbiome of medicinal plants in different tissues with their growth. In this study, we examined the endophytic bacterial and fungal community structures associated with both the stem and root compartments of Dendrobium huoshanense at different growth years via high-throughput sequencing of 16S rRNA genes and nrDNA fragments of internal transcribed spacer regions. Results indicated that more diverse prokaryotic and fungal operational taxonomic units were detected in roots than in stems, and the alpha diversity of endophytic prokaryotic significantly differed among the 1-, 2-, and 3-year-old roots. The dominant bacterial phyla Proteobacteria Firmicutes, Actinobacteria, Bacteroidetes, and Acidobacteria, and fungal phyla Ascomycota, Basidiomycota, and Ascomycota were detected in the stems and roots with 3 growth years. Moreover, linear discriminant effect size analysis revealed 138 differentially abundant taxonomic clades in the bacterial level, and 197 in the fungal level in six groups. Our results provide evidence for endophytic microbiota communities depending on the tissues and growth years of D. huoshanense. The results from this study should be useful to better understand medicinal plant-microbe interactions.

中文翻译：

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不同生育年间霍山石斛根茎内生菌群比较

药用植物内生微生物组丰富多样，但很少有研究跟踪药用植物不同组织中的内生微生物组及其生长。在这项研究中，我们通过对 16S rRNA 基因和内部转录间隔区的 nrDNA 片段进行高通量测序，研究了与不同生长年份霍山石斛茎和根区室相关的内生细菌和真菌群落结构。结果表明，在根中检测到的原核和真菌操作分类单元比在茎中更多样化，并且内生原核的 α 多样性在 1 年、2 年和 3 年生根中存在显着差异。优势细菌门 Proteobacteria Firmicutes、Actinobacteria、Bacteroidetes 和 Acidobacteria，以及真菌门 Ascomycota，在生长3年的茎和根中检测到担子菌和子囊菌。此外，线性判别效应大小分析显示，六组在细菌水平上有 138 个差异丰富的分类进化枝，在真菌水平上有 197 个差异丰富的分类进化枝。我们的研究结果为内生微生物群落提供了证据，这取决于霍山红豆杉的组织和生长年限。这项研究的结果应该有助于更好地了解药用植物与微生物的相互作用。火山人。这项研究的结果应该有助于更好地了解药用植物与微生物的相互作用。火山人。这项研究的结果应该有助于更好地了解药用植物与微生物的相互作用。