We describe a nanocomposite material for the electrochemical detection of β -nicotinamide adenine dinucleotide (NADH), a coenzyme involved in redox reactions of all living cells and in the detection of many organic species by electrochemical biosensors. The composite is made of nanosheets of electrochemically exfoliated graphene oxide (EGO) covalently functionalized with dopamine (DP) molecules. The EGO DP material finally obtained is rich of 1,2-dihydroxyphenyl moieties and is able to detect NADH at a particular low potential value with higher sensitivity with respect to pristine EGO. To study the effectiveness of 1,2-dihydroxyphenyl moieties in inducing electrocatalytic oxidation of NADH, we combined standard voltammetric techniques with UV–Vis absorption spectroelectrochemistry, which allowed us to measure the variations in composition occurring at the electrode|solution interface, i.e. to measure the consumption rate of NADH. Spectroelectrochemi...

中文翻译：

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多巴胺官能化的氧化石墨烯作为生物传感的高性能材料

我们描述了一种用于电化学检测β-烟酰胺腺嘌呤二核苷酸（NADH）的纳米复合材料，这是一种参与所有活细胞的氧化还原反应并通过电化学生物传感器检测许多有机物的辅酶。该复合材料由多巴胺（DP）分子共价官能化的电化学剥离氧化石墨烯（EGO）纳米片制成。最终获得的EGO DP材料富含1,2-二羟基苯基部分，并且能够以相对于原始EGO更高的灵敏度检测到特定的低电位值的NADH。为了研究1,2-二羟基苯基部分诱导NADH电催化氧化的有效性，我们将标准伏安技术与UV-Vis吸收光谱电化学相结合，由此，我们可以测量在电极界面上发生的组成变化，即可以测量NADH的消耗率。光谱电化学...