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Catheter-related bloodstream infection due to Tsukamurella pulmonis identified by MALDI-TOF spectrometry, 16S rRNA gene sequencing, and secA1 gene sequencing in an immunocompromised child: a case report and literature review.
Diagnostic Microbiology and Infectious Disease ( IF 2.9 ) Pub Date : 2020-03-29 , DOI: 10.1016/j.diagmicrobio.2020.115052
A G Leroy 1 , E Persyn 1 , A Guillouzouic 1 , L Ruffier d'Epenoux 1 , E Launay 2 , E-M Takoudju 3 , M-E Juvin 1 , F Chantreau 1 , J El Khobzi 1 , P Bémer 1 , S Corvec 1
Affiliation  

Tsukamurella species are Gram-positive bacilli related to aerobic Actinomyces. Originally reported from the environment, Tsukamurella species have also been described in human infections, especially in bacteremia. A literature review analysis revealed that Tsukamurella spp. are often initially considered as contaminant microorganisms, especially due to bacterial identification issues. Here, we report a catheter-related bloodstream infection in an immunocompromised child caused by Tsukamurella pulmonis. Matrix-Assisted Laser Desorption/Ionization-Time Of Flight (MALDI-TOF) mass spectrometry allowed rapid genus-level identification and contributed to better patient care. However, accurate species-level identification required 16S rRNA gene sequencing and secA1 gene sequencing. Considering the increased number of Tsukamurella infections, the implementation of new Tsukamurella species in MALDI-TOF databases is required to be more discriminant.

中文翻译:

MALDI-TOF光谱法,16S rRNA基因测序和secA1基因测序在免疫功能低下的儿童中识别出由于肺炎衣原体引起的导管相关血流感染:病例报告和文献复习。

冢卡氏菌属与需氧放线菌有关的革兰氏阳性杆菌。最初从环境中报道,冢卡氏菌属菌种也已在人类感染,尤其是菌血症中描述。文献综述分析表明,冢卡氏菌属。最初通常被视为污染物微生物,尤其是由于细菌识别问题。在这里,我们报告了由肺部冢卡氏菌引起的免疫受损儿童的导管相关血流感染。基质辅助激光解吸/电离飞行时间(MALDI-TOF)质谱技术可快速鉴定属水平,并有助于改善患者护理水平。但是,准确的物种水平鉴定需要16S rRNA基因测序和secA1基因测序。考虑到Tsukamurella感染数量增加,
更新日期:2020-04-20
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