Salivary antibodies are useful in surveillance and vaccination studies. However, low antibody levels and degradation by endonucleases are problematic. Oral flocked swabs are a potential non-invasive alternative for detecting viral antibodies. Seroprevalence for Cytomegalovirus (CMV), Varicella-Zoster virus (VZV), Epstein-Barr virus (EBV), Measles and Mumps IgG antibodies were determined from 50 matched serum, saliva and swabs samples from healthy volunteers using commercial ELISAs. CMV IgG, VZV IgG, and EBV EBNA-1 IgG, VCA IgG, and Measles IgG swab versus serum sensitivities were 95.8%, 96.0%, 92.1%, 95.5%, 84.5%, respectively, and swabs correlated well with saliva. Sensitivity of Mumps IgG in swabs and saliva was poor at 60.5%, and 68.2%, respectively. Specificities for IgG antibodies were 100% for CMV, EBV and Mumps, but could not be determined for VZV and Measles due to exclusively seropositive volunteers. Except for Mumps IgG, swabs correlate well with serum, are easy to self-collect and are stable at room temperature.

中文翻译：

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使用植绒口腔拭子非侵入性检测常见病原性病毒的IgG抗体。

唾液抗体可用于监测和疫苗接种研究。然而，低抗体水平和核酸内切酶的降解是有问题的。植绒口腔拭子是检测病毒抗体的潜在非侵入性替代方法。巨细胞病毒（CMV），水痘-带状疱疹病毒（VZV），爱泼斯坦-巴尔病毒（EBV），麻疹和腮腺炎的血清阳性率使用商业ELISA从50名健康志愿者的匹配血清，唾液和拭子样本中确定IgG抗体。CMV IgG，VZV IgG和EBV EBNA-1 IgG，VCA IgG和麻疹IgG拭子对血清的敏感性分别为95.8％，96.0％，92.1％，95.5％，84.5％，并且拭子与唾液的相关性很好。腮腺炎IgG在拭子和唾液中的敏感性较差，分别为60.5％和68.2％。IgG抗体对CMV，EBV和腮腺炎的特异性为100％，但由于是血清反应阳性的自愿者，因此无法确定VZV和麻疹。除腮腺炎IgG外，拭子与血清具有良好的相关性，易于自我收集并且在室温下稳定。