In this study, we described the largest analysis to date conducted with VIDAS® HIV Duo Ultra assay. Additionally, we analyzed the diagnostic performance and cutoff values (TV) of HIV Duo Ultra assay and total cost analysis for HIV testing. Of 11,642 enzyme-linked immunosorbent assay (ELISA)–positive samples referred to our center for confirmation, 2000 were positive with HIV Duo Ultra, and of these, 87% were HIV-1 positive and 0.6% were HIV-1 indeterminate with the confirmatory test. Overall, the false-positivity rate was 1.75% for HIV Duo Ultra assay. The sensitivity and specificity were 100% and 99.1%, respectively, when the TV was set at the recommended cutoff value. Even increasing the cutoff value four times, sensitivity and specificity remained high, pointing out that a TV of 0.99 is highly indicative of HIV positivity. Retesting samples with HIV Duo Ultra assay decreased 80% of the confirmatory tests, revealing a significant decrease of 78% in the total costs and reporting time.

在这项研究中，我们描述了迄今为止使用VIDAS®HIV Duo Ultra分析进行的最大的分析。此外，我们分析了HIV Duo Ultra检测的诊断性能和临界值（TV），以及HIV检测的总成本分析。在转交给我们中心进行确认的11,642种酶联免疫吸附测定（ELISA）阳性样品中，2000例HIV Duo Ultra呈阳性，其中87％的HIV-1阳性和0.6％的HIV-1不确定。测试。总体而言，HIV Duo Ultra检测的假阳性率为1.75％。当将电视设置为推荐的临界值时，灵敏度和特异性分别为100％和99.1％。即使将临界值提高了四倍，敏感性和特异性仍然很高，指出0.99的电视高度表明HIV阳性。