Resveratrol, a well-known antioxidant, has been reported to protect mouse metaphase-II (M - II) stage oocytes from vitrification injuries when used as a treatment during a series of vitrification processes. The present study was conducted to investigate whether short-term treatment of post-warm bovine mature oocytes with resveratrol can increase blastocyst formation rate following in vitro fertilization and culture. Bovine denuded M - II oocytes were vitrified-warmed using Cryotop® or nylon mesh (pore size = 37 μm) as a cryodevice. The post-warm oocytes were treated for 2 h with 1 μM resveratrol in recovery culture medium. The resveratrol treatment had no harmful influence on morphological survival and cleavage rate of the oocytes vitrified-warmed with Cryotop® or nylon mesh. In the Cryotop® vitrification series, blastocyst formation rate of resveratrol-treated post-warm oocytes (39.0%) was not significantly different from that of non-treated post-warm oocytes (31.7%). However in the nylon mesh vitrification series, there was a significant increase in the blastocyst yield (42.4% vs. 31.3%, P < 0.05) when post-warm oocytes were treated with resveratrol. Blastocyst yield from fresh control oocytes was 49%. Levels of reactive oxygen species were comparable between post-warm and fresh control M - II oocytes, and decreased in oocytes after recovery culture with resveratrol. Mitochondrial activity of post-warm oocytes was restored to the pre-vitrification level during the recovery culture regardless of resveratrol supplementation. Thus, short-term recovery culture with resveratrol can rescue bovine M - II oocytes vitrified-warmed on a nylon mesh cryodevice.

中文翻译：

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白藜芦醇短期恢复培养抢救玻璃化加热牛成熟卵母细胞

据报道，众所周知的抗氧化剂白藜芦醇在一系列玻璃化过程中用作治疗时可保护小鼠中期 II (M-II) 期卵母细胞免受玻璃化损伤。本研究旨在调查用白藜芦醇短期处理温后牛成熟卵母细胞是否可以增加体外受精和培养后的囊胚形成率。使用 Cryotop® 或尼龙网（孔径 = 37 μm）作为冷冻装置对牛去核的 M-II 卵母细胞进行玻璃化加热。加热后的卵母细胞在恢复培养基中用 1 μM 白藜芦醇处理 2 小时。白藜芦醇处理对用 Cryotop® 或尼龙网玻璃化加热的卵母细胞的形态存活和卵裂率没有有害影响。在 Cryotop® 玻璃化系列中，白藜芦醇处理的温后卵母细胞的囊胚形成率（39.0%）与未处理的温后卵母细胞（31.7%）没有显着差异。然而，在尼龙网玻璃化系列中，当用白藜芦醇处理加热后的卵母细胞时，囊胚产量显着增加（42.4% 对 31.3%，P < 0.05）。新鲜对照卵母细胞的囊胚产量为 49%。温暖后和新鲜对照 M-II 卵母细胞的活性氧水平相当，并且在用白藜芦醇恢复培养后卵母细胞中的活性氧水平降低。无论是否添加白藜芦醇，加热后卵母细胞的线粒体活性在恢复培养期间恢复到玻璃化前水平。因此，用白藜芦醇进行短期恢复培养可以挽救在尼龙网冷冻装置上玻璃化加热的牛 M-II 卵母细胞。