The endogenous opioid peptides have been reported to be involved in the regulation of reproductive physiology. Many of the studies conclude with sentences around the harmful effect of opioids in male fertility but, actually, there is only one study regarding the real fertility potential of spermatozoa that have been exposed to mu specific opioids. The aim of the present study was to see if the modulation of delta (OPRD1) and kappa (OPRK1) opioid receptors in mouse sperm during capacitation was able to vary the embryo production after in vitro fertilization (IVF). The presence of OPRD1 and OPRK1 in mouse mature spermatozoa was analyzed by RT-PCR and immunofluorescence. Incubating the sperm with, on one hand, the delta specific agonist DPDPE and/or antagonist naltrindole, and, on the other hand, the kappa specific agonist U-50488 and antagonist nor-binaltorphimine, we analyzed the involvement of OPRD1 and OPRK1 on IVF and preimplantational embryo development. We verified the presence of OPRD1 and OPRK1 in mouse mature spermatozoa, not only at the mRNA level but also at protein level. Moreover, the sperm incubation with DPDPE, before the IVF, had an effect on the fertilization rate of sperm and reduced the number of reached blastocysts, which was reverted by naltrindole. Instead, the use of the kappa agonist U-50488 and the antagonist nor-binaltophimine did not have any effect on the amount and the quality of the achieved blastocysts. Although nowadays the pure delta or kappa opioid ligands are not used for the clinic, clinical trials are being conducted to be used in the near future, so it would be interesting to know if the modulation of these receptors in sperm would generate any consequence in relation to fertilization capacity.

据报道内源性阿片样物质肽参与生殖生理的调节。许多研究以关于阿片类药物对男性生育的有害影响的句子作为结尾，但实际上，只有一项研究涉及暴露于μ特定阿片类物质的精子的实际生育潜力。本研究的目的是，以查看是否增量的调制（OPRD1）和κ（OPRK1）能过程阿片受体在小鼠精子是能够改变后的胚胎生产的体外受精（IVF）。通过RT-PCR和免疫荧光分析小鼠成熟精子中OPRD1和OPRK1的存在。一方面，将精子与δ特异性激动剂DPDPE和/或拮抗剂纳曲酮一起孵育，另一方面与κ特异性激动剂U-50488和拮抗剂去甲双萘酚胺一起孵育，我们分析了OPRD1和OPRK1对IVF的参与和植入前的胚胎发育。我们验证了小鼠成熟精子中不仅在mRNA水平而且在蛋白质水平存在OPRD1和OPRK1。此外，在体外受精之前，用DPDPE孵育精子对精子的受精率有影响，并减少了到达的胚泡数，而纳曲酮可逆转胚泡。代替，使用Kappa激动剂U-50488和拮抗剂去甲倍萘啶对获得的胚泡的数量和质量没有任何影响。尽管如今，纯三角洲或κ阿片样物质配体尚未用于临床，但正在进行临床试验以在不久的将来使用，因此，了解这些受体在精子中的调节是否会产生任何相关的结果将很有趣。施肥能力。