Being aware of the remarkable antimicrobial potential of S. officinalis L., we aimed to evaluate the antimicrobial activity of the S. officinalis dichloromethane crude extract (SOD), dichloromethane-soluble fractions (SODH and SODD), SODD subfractions (SODD1 and SODD2), and pure substances (manool, salvigenin, and viridiflorol) against periodontopathogens. This bioassay-guided study comprises five antimicrobial tests—determination of the Minimum Inhibitory Concentration (MIC), determination of the Minimum Bactericidal Concentration (MBC), determination of the antibiofilm activity, construction of the Time-kill curve (determination of Bactericidal Kinetics), and determination of the Fractional Inhibitory Concentration Index—on six clinical bacterial isolates and three standard bacterial strains involved in periodontal disease. SOD has moderate activity against most of the tested bacteria, whereas SODD1, SODH1, SODH3, and manool afford the lowest results. The Porphyromonas gingivalis (ATTC and clinical isolate) biofilm is considerably resistant to all the samples. In association with chlorhexidine gluconate, only SODH1 exerts additive action against P. gingivalis (clinical isolate). Therefore, SODH1 and manool are promising antibacterial agents and may provide therapeutic solutions for periodontal infections.

意识到沙门氏菌具有显着的抗菌潜力，我们旨在评估沙门氏菌的抗菌活性。二氯甲烷粗提物（SOD），可溶于二氯甲烷的馏分（SODH和SODD），SODD子馏分（SODD1和SODD2）以及针对牙周病原体的纯净物质（manool，salvigenin和viridiflorol）。这项由生物测定法指导的研究包括5种抗菌药物测试：最小抑菌浓度（MIC）的测定，最小杀菌浓度（MBC）的测定，抗生物膜活性的测定，时间杀伤曲线的构建（杀菌动力学的测定），并测定了六种临床牙菌和三种与牙周病有关的标准细菌菌株的抑制分数。SOD对大多数被测细菌具有中等活性，而SODD1，SODH1，SODH3和马诺酚的结果最低。的牙龈卟啉单胞菌（ATTC和临床分离株）生物膜对所有样品均具有相当的抵抗力。与葡萄糖酸氯己定联合使用时，只有SODH1对牙龈卟啉单胞菌（临床分离株）发挥加和作用。因此，SODH1和马诺酚是有前途的抗菌剂，可以为牙周感染提供治疗解决方案。