The lethal chronic airway infection of the cystic fibrosis (CF) patients is predisposed by colonization of specific CF-philic pathogens or the CF microbiomes, but key processes and reasons of the microbiome settlement in the patients are yet to be fully understood, especially their survival and metabolic dynamics from normal to diseased status under treatment. Here, we report our meta-analysis results on CF airway microbiomes based on metabolic networks reconstructed from genome information at species level. The microbiomes of CF patients appear to engage much more redox-related activities than those of controls, and by constructing a large dataset of anti-oxidative stress (anti-OS) genes, our quantitative evaluation of the anti-OS capacity of each bacterial species in the CF microbiomes confirms strong conservation of the anti-OS responses within genera and also shows that the CF pathogens have significantly higher anti-OS capacity than commensals and other typical respiratory pathogens. In addition, the anti-OS capacity of a relevant species correlates with its relative fitness for the airways of CF patients over that for the airways of controls. Moreover, the total anti-OS capacity of the respiratory microbiome of CF patients is collectively higher than that of controls, which increases with disease progression, especially after episodes of acute exacerbation and antibiotic treatment. According to these results, we propose that the increased OS in the airways of CF patients may play an important role in reshaping airway microbiomes to a more resistant status that favors the pre-infection colonization of the CF pathogens for a higher anti-OS capacity.

中文翻译：

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荟萃分析揭示了氧化应激对囊性纤维化患者气道微生物的潜在影响。

囊性纤维化（CF）患者的致死性慢性气道感染是由特定的CF亲病性病原体或CF微生物群定植而引起的，但是患者中微生物组沉降的关键过程和原因尚待充分了解，尤其是它们的存活率以及从正常状态到患病状态的代谢动力学。在这里，我们报告了基于基于物种水平的基因组信息重建的代谢网络的CF气道微生物群的荟萃分析结果。CF患者的微生物组似乎比对照组具有更多的氧化还原相关活性，并且通过构建大量的抗氧化应激（anti-OS）基因数据集，我们对CF微生物群中每种细菌的抗OS能力的定量评估证实了属内抗OS反应的强烈保守性，并且还表明CF病原体的抗OS能力明显高于普通细菌和其他典型呼吸道病原体。另外，相关物种的抗OS能力与其对CF患者气道的相对适应度相对于对照气道的相对适应度相关。此外，CF患者的呼吸微生物组的总抗OS能力总体上高于对照，这随疾病进展而增加，尤其是在急性加重发作和抗生素治疗后。根据这些结果，