In this study, a factorial experiment was carried out to investigate the effect of AMF (arbuscular mycorrhizal fungi) and water stress on the quality of fresh plants (through the analysis of essential oil composition) and also on the morphological characteristics (in terms of biomass) of basil (Ocimum basilicum L. cv. Genovese Gigante). The factors included an arbuscular mycorrhizal fungus inoculation (Glomus intraradices), irrigation level (mild water stress, 60% Field capacity) and the interaction of both AMF and water stress. In addition, the effects of harvesting at two different developmental stages were assessed with respect to the plants tolerance to abiotic stress and the quality of the fresh plant. The main essential oil (EO) constituents for this cultivar were eugenol, which varied between 7.1 and 50.8%, and linalool, 17.0–54.7%, in all the samples. The highest relative amount of eugenol (50.8%) was obtained during the vegetative stage in plants under water stress whereas samples from the control presented 16.2% for this compound. The samples which were significantly different from the control samples were the WS (water stress) samples from the vegetative stage. Additionally, this study suggests that AMF plants offset the adverse effect of water stress. In this context, and since the interaction of AMF and water stress did not significantly change the fresh plant quality, i.e. the essential oil profile in both stages of development, it is worthwhile considering this method of production. Finally, this experiment permitted an understanding of the stressor thresholds of the species used and also that, compensatory responses occur at different time scales, including between generations and also during development (developmental plasticity).

中文翻译：

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Ocimum basilicum L. cv. 精油的成分变化 Genovese Gigante 在不同生长阶段对 Glomus intraradices 和轻度水分胁迫的响应

在这项研究中，进行了一项析因实验，以研究 AMF（丛枝菌根真菌）和水分胁迫对新鲜植物品质（通过精油成分分析）和形态特征（生物量方面）的影响。 ) 罗勒 (Ocimum basilicum L. cv. Genovese Gigante)。这些因素包括丛枝菌根真菌接种（Glomus intraradices）、灌溉水平（轻度水分胁迫，60% 田间持水量）以及 AMF 和水分胁迫的相互作用。此外，还评估了在两个不同发育阶段收获对植物对非生物胁迫的耐受性和新鲜植物质量的影响。该品种的主要精油 (EO) 成分是丁香酚，含量在 7.1% 至 50.8% 之间，芳樟醇含量在 17.0% 至 54.7% 之间，在所有样本中。在水分胁迫下植物的营养阶段获得了最高的丁香酚相对量 (50.8%)，而对照样品中该化合物的含量为 16.2%。与对照样品显着不同的样品是来自营养阶段的WS（水分胁迫）样品。此外，这项研究表明 AMF 植物抵消了水分胁迫的不利影响。在这种情况下，由于 AMF 和水分胁迫的相互作用并没有显着改变新鲜植物的质量，即两个开发阶段的精油分布，值得考虑这种生产方法。最后，该实验允许了解所用物种的压力源阈值，以及在不同时间尺度上发生的补偿反应，