Abstract In recent years we initiated extensive studies on the characterization of the population structure of wild Lactuca relatives (WLRs) originating from their center of origin and diversity in Southwest Asia. A comparative phytochemical study of nine sesquiterpene lactones in natural populations of the wild lettuce L. altaica Fisch. & C.A. Mey. (Asteraceae) was performed, based on 22 plants, representing seven original individual seed samples derived from three localities representing three regions in Armenia. The compounds were profiled and quantified in leaves and roots of the plants, grown in a controlled glasshouse. The contents of major sesquiterpene lactones, that including the following eight guaianolides: cichorioside B, lactucin, 11β,13-dihydrolactucin, crepidiaside B, 8-deoxylactucin, jacquinelin, lactucopicrin/11β,13-dihydrolactucopicrin, as well as the germacranolide glucoside – lactuside A, were estimated by HPLC/PDA. The L. altaica plants could be characterized by the occurrence of lactuside A in their roots, and the mixture of lactucopicrin/11β,13-dihydrolactucopicrin in both their roots and leaves by relatively high amounts, similarly to results obtained for three commercial cultivars of L. sativa. The total content of sesquiterpene lactones in the roots was significantly higher than that in the leaves. This study is likely the first report of detailed screening of L. altaica natural populations and individuals, even by low sample size, for any trait. Species within the primary lettuce gene pool, should be considered as an attractive source of germplasm in further research and improvement of cultivated lettuce, Lactuca sativa L. While using interspecific hybridization in order to elevate the sesquiterpene levels in cultivated lettuces, the lactones quality (profile) and quantity, as well as the cross-ability level of the wild Lactuca spp. with the crop and fertility of the obtained offspring should be considered.

中文翻译：

---

亚美尼亚Lactuca altaica自然种群倍半萜内酯含量的变化

摘要 近年来，我们对源自西南亚起源和多样性中心的野生莴苣近缘种 (WLR) 的种群结构特征进行了广泛的研究。野生生菜 L. altaica Fisch 自然种群中九种倍半萜内酯的比较植物化学研究。＆CA 梅。（菊科）基于 22 株植物进行，代表来自代表亚美尼亚三个地区的三个地点的七个原始个体种子样本。在受控温室中生长的植物的叶子和根中对化合物进行了分析和量化。主要倍半萜内酯的含量，包括以下八种愈创木酚内酯：菊苣甙B、乳聚糖苷、11β,13-二氢乳聚糖苷、crepidiaside B、8-脱氧乳聚糖苷、jacquinelin、lactucopicrin/11β、13-二氢乳苦苦苷，以及germacranolide glucoside – lactuside A，通过HPLC/PDA 估计。L. altaica 植物的特征在于它们的根中存在乳苦苷 A，并且它们的根和叶中的乳苦苷/11β,13-二氢乳苦苷的混合物的含量相对较高，类似于 L. 的三个商业品种获得的结果. 苜蓿。根中倍半萜内酯的总含量显着高于叶中。这项研究可能是第一份详细筛选 L. altaica 自然种群和个体的报告，即使样本量很小，也可以针对任何性状进行筛选。初级生菜基因库中的物种应被视为进一步研究和改良栽培生菜 Lactuca sativa L 的有吸引力的种质来源。在使用种间杂交以提高栽培生菜中倍半萜烯水平的同时，内酯的质量（特征）和数量，以及野生莴苣属的杂交能力水平。应考虑获得后代的作物和生育能力。