A field indoor air measurement of SARS-CoV-2 in the patient rooms of the largest hospital in Iran.,Science of the Total Environment

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A field indoor air measurement of SARS-CoV-2 in the patient rooms of the largest hospital in Iran.
Science of the Total Environment ( IF 9.8 ) Pub Date : 2020-04-06 , DOI: 10.1016/j.scitotenv.2020.138401
Sasan Faridi ₁ , Sadegh Niazi ₂ , Kaveh Sadeghi ₃ , Kazem Naddafi ₁ , Jila Yavarian ₃ , Mansour Shamsipour ₄ , Nazanin Zahra Shafiei Jandaghi ₃ , Khosro Sadeghniiat ₅ , Ramin Nabizadeh ₁ , Masud Yunesian ₁ , Fatemeh Momeniha ₆ , Adel Mokamel ₇ , Mohammad Sadegh Hassanvand ₈ , Talat MokhtariAzad ₃

Affiliation

Centre for Air Pollution Research (CAPR), Institute for Environmental Research (IER), Tehran University of Medical Sciences, Tehran, Iran; Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
International Laboratory for Air Quality and Health, School of Earth and Atmospheric Sciences, Science and Engineering Faculty, Queensland University of Technology (QUT), Brisbane, Queensland, Australia.
Department of Virology, School of Public Health, Tehran University of Medical Sciences.
Department of Research Methodology and Data Analysis, Institute for Environmental Research, Tehran University of Medical Sciences, Tehran, Iran.
Occupational Sleep Research Center, Baharloo Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Department of Environmental Health Engineering, School of Public Health, Iran University of Medical Sciences, Tehran, Iran.
Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Centre for Air Pollution Research (CAPR), Institute for Environmental Research (IER), Tehran University of Medical Sciences, Tehran, Iran.

The coronavirus disease 2019 (COVID-19) emerged in Wuhan city, China, in late 2019 and has rapidly spread throughout the world. The major route of transmission of SARS-CoV-2 is in contention, with the airborne route a likely transmission pathway for carrying the virus within indoor environments. Until now, there has been no evidence for detection of airborne severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and this may have implication for the potential spread of the COVID-19. We investigated the air of patient rooms with confirmed COVID-19 in the largest hospital in Iran, on March 17, 2020. To collect the SARS-CoV-2 particles, ten air samples were collected into the sterile standard midget impingers containing 20 mL DMEM with 100 μg/mL streptomycin, 100 U/mL penicillin and 1% antifoam reagent for 1 h. Besides, indoor particle number concentrations, CO2, relative humidity and temperature were recorded throughout the sampling duration. Viral RNA was extracted from samples taken from the impingers and Reverse-Transcription PCR (RT-PCR) was applied to confirm the positivity of collected samples based on the virus genome sequence. Fortunately, in this study all air samples which were collected 2 to 5 m from the patients' beds with confirmed COVID-19 were negative. Despite we indicated that all air samples were negative, however, we suggest further in vivo experiments should be conducted using actual patient cough, sneeze and breath aerosols in order to show the possibility of generation of the airborne size carrier aerosols and the viability fraction of the embedded virus in those carrier aerosols.

更新日期：2020-04-08

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