Recently a species of Pseudanabaena was identified as the dominant photosynthetic organism during a bloom event in a high pH (pH ∼11.4), radioactive spent nuclear fuel pond (SNFP) at the Sellafield Ltd., United Kingdom facility. The metabolic response of a laboratory culture containing the cyanobacterium Pseudanabaena catenata, a relative of the major photosynthetic microorganism found in the SNFP, to X-ray irradiation was studied to identify potential survival strategies used to support colonization of radioactive environments. Growth was monitored and the metabolic fingerprints of the cultures, during irradiation and throughout the post-irradiation recovery period, were determined using Fourier transform infrared (FT-IR) spectroscopy. A dose of 95 Gy delivered over 5 days did not significantly affect growth of P. catenata, as determined by turbidity measurements and cell counts. Multivariate statistical analysis of the FT-IR spectral data revealed metabolic variation during the post-irradiation recovery period, with increased polysaccharide and decreased amide spectral intensities. Increases in polysaccharides were confirmed by complementary analytical methods including total carbohydrate assays and calcofluor white staining. This observed increased production of polysaccharides is of significance, since this could have an impact on the fate of the radionuclide inventory in the pond via biosorption of cationic radionuclides, and may also impact on downstream processes through biofilm formation and biofouling.

中文翻译：

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与第一代 Magnox 存储池相关的蓝藻 Pseudanabaena catenata 的辐射耐受性。

最近，在英国塞拉菲尔德有限公司的放射性废核燃料池 (SNFP) 的高 pH 值（pH ∼ 11.4）的水华事件中，一种 Pseudanabaena 被确定为主要的光合生物。研究了含有蓝藻链状蓝藻（SNFP 中发现的主要光合微生物的近亲）的实验室培养物对 X 射线照射的代谢反应，以确定用于支持放射性环境定植的潜在生存策略。监测生长，并使用傅里叶变换红外（FT-IR）光谱测定培养物在辐照期间和整个辐照后恢复期间的代谢指纹。根据浊度测量和细胞计数确定，5 天内施加 95 Gy 的剂量不会显着影响链状 P. catenata 的生长。FT-IR 光谱数据的多变量统计分析揭示了辐照后恢复期间的代谢变化，多糖增加而酰胺光谱强度降低。多糖的增加通过补充分析方法（包括总碳水化合物测定和卡尔科弗洛尔白染色）证实。观察到的多糖产量增加具有重要意义，因为这可能通过阳离子放射性核素的生物吸附对池塘中放射性核素库存的命运产生影响，并且还可能通过生物膜形成和生物污垢对下游过程产生影响。