Canine coronavirus (CCoV) strains with the ability to spread to internal organs, also known as pantropic CCoVs (pCCoVs), have been detected in domestic dogs and wild carnivores. Our study focused on the detection and molecular characterization of pCCoV strains circulating in Italy during the period 2014–2017 in autochthonous dogs, in dogs imported from eastern Europe or illegally imported from an unknown country. Samples from the gut and internal organs of 352 dogs were screened for CCoV; putative pCCoV strains, belonging to subtype CCoV‐IIa, were identified in the internal organs of 35 of the examined dogs. Fifteen pCCoV strains were subjected to sequence and phylogenetic analyses, showing that three strains (98960‐1/2016, 98960‐3/2016, 98960‐4/2016) did not cluster either with Italian or European CCoVs, being more closely related to alphacoronaviruses circulating in Asia with which they displayed a 94%–96% nucleotide identity in partial spike protein gene sequences. The pCCoV‐positive samples were also tested for other canine viruses, showing co‐infections mainly with canine parvovirus.

中文翻译：

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意大利本土和进口狗中泛热带犬冠状病毒的传播。

已在家犬和野生食肉动物中检测到具有传播至内脏器官能力的犬冠状病毒（CCoV）菌株，也称为泛嗜性 CCoV（pCCoV）。我们的研究重点是 2014 年至 2017 年期间在意大利本土犬只、从东欧进口或从未知国家非法进口的犬只中传播的 pCCoV 毒株的检测和分子特征分析。对 352 只狗的肠道和内脏样本进行了 CCoV 筛查；在 35 只接受检查的狗的内脏器官中发现了推定的 pCCoV 毒株，属于 CCoV-IIa 亚型。对 15 种 pCCoV 病毒株进行了序列和系统发育分析，结果显示，其中 3 种病毒株（98960-1/2016、98960-3/2016、98960-4/2016）既不与意大利或欧洲 CCoV 聚类，而是与 α 冠状病毒关系更密切。在亚洲流行，它们在部分刺突蛋白基因序列中显示出 94%–96% 的核苷酸同一性。pCCoV 阳性样本还进行了其他犬病毒检测，显示主要与犬细小病毒合并感染。