The aim of this study was to investigate the influence of surface hydrophobicity and roughness of carrier materials on the inactivation of bacterial spores with gaseous hydrogen peroxide whereas condensate formation is prevented. Spores of Bacillus subtilis and Bacillus atrophaeus were applied either as single spore culture or as a mixed spore population to simulate natural contamination with microorganisms of different characteristics. Inactivation with gaseous hydrogen peroxide was carried out at 5200 ppm hydrogen peroxide without condensate formation. The inactivation results of B. subtilis and B. atrophaeus spores on carrier materials with varying surface hydrophobicity differed significantly. However, inactivation of the mixed spore populations resulted in similar resistance compared to the single spore batches. The results of this study indicate that surface hydrophobicity most probably has an impact on the inactivation with gaseous hydrogen peroxide whereas surface roughness only plays a minor role.

中文翻译：

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汽化过氧化氢对固体表面的孢子灭活-载体材料表面特性的影响

本研究的目的是研究表面疏水性和载体材料的粗糙度对用气态过氧化氢灭活细菌孢子的影响，同时防止冷凝物形成。枯草芽孢杆菌和萎缩芽孢杆菌的孢子作为单个孢子培养物或作为混合孢子种群应用，以模拟不同特征微生物的自然污染。用气态过氧化氢灭活在 5200 ppm 过氧化氢下进行，没有形成冷凝物。枯草芽孢杆菌和萎缩芽孢杆菌孢子在具有不同表面疏水性的载体材料上的灭活结果显着不同。然而，与单个孢子批次相比，混合孢子种群的灭活导致相似的抗性。