The implementation of Integrated Pest Management in current agricultural practice is a convenient and very effective strategy to keep pest populations under control. The use of biological control agents, such as Phytoseiulus persimilis, is key for the success of such an approach. This predatory mite is widely used as it is very effective for controlling Tetranychus urticae, one of the most devastating crop pests. Here, we identify several mutations located in the voltage‐gated sodium channel (VGSC) of commercially sourced P. persimilis that correlate with a reduced susceptibility to the pyrethroid deltamethrin. We found that the mites sourced from two different biocontrol product companies have intrinsic genotypic differences that correlate with their phenotype when tested with different concentrations of deltamethrin. Mites from Syngenta Bioline, carrying the mutations M918L and A1536T, were able to survive deltamethrin concentrations of up to 10 ppm, while the mites from Koppert Biological Systems, with the combination M918L, L925V and S1539T, survived treatment with 40 ppm. All of the point mutations identified in the predatory mite samples are located in a particular region of the VGSC, previously proposed as the binding site for this family of pesticides and identified as a ‘hot spot’ for resistance.

中文翻译：

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在商业来源的百草枯草中，与氯氰菊酯抗性相关的电压门控钠通道基因突变。

在当前的农业实践中实施病虫害综合治理是使病虫害种群得到控制的便捷而有效的战略。使用生物防治剂，例如柿树（Phytoseiulus persimilis），是这种方法成功的关键。这种捕食性螨由于对防治最具破坏性的农作物害虫之一的四叶螨非常有效，因此被广泛使用。在这里，我们确定了位于商业来源的柿叶假单胞菌的电压门控钠通道（VGSC）中的几个突变与降低对拟除虫菊酯溴氰菊酯的敏感性有关。我们发现，当使用不同浓度的溴氰菊酯测试时，来自两个不同生物防治产品公司的螨虫具有内在的基因型差异，这些差异与它们的表型相关。具有先天突变的M918L和A1536T突变的螨虫能够在溴氰菊酯浓度高达10 ppm的条件下存活，而Koppert Biological Systems的螨虫结合M918L，L925V和S1539T的螨虫能够以40 ppm的剂量存活。在捕食性螨样品中鉴定出的所有点突变都位于VGSC的特定区域，该区域先前被提议为该农药家族的结合位点，并被确定为抗药性的“热点”。