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Nanoparticle-Based Platform for Activatable Fluorescence Imaging and Photothermal Ablation of Endometriosis.
Small ( IF 13.3 ) Pub Date : 2020-04-06 , DOI: 10.1002/smll.201906936
Abraham S Moses 1 , Olena R Taratula 1 , Hyelim Lee 1 , Fangzhou Luo 2 , Tanner Grenz 2 , Tetiana Korzun 1 , Anna St Lorenz 1 , Fahad Y Sabei 1 , Shay Bracha 3 , Adam W G Alani 1 , Ov D Slayden 2 , Oleh Taratula 1
Affiliation  

Endometriosis is a painful disorder where endometrium-like tissue forms lesions outside of the uterine cavity. Intraoperative identification and removal of these lesions are difficult. This study presents a nanoplatform that concurrently delineates and ablates endometriosis tissues using real-time near-infrared (NIR) fluorescence and photothermal therapy (PTT). The nanoplatform consists of a dye, silicon naphthalocyanine (SiNc), capable of both NIR fluorescence imaging and PTT, and a polymeric nanoparticle as a SiNc carrier to endometriosis tissue following systemic administration. To achieve high contrast during fluorescence imaging of endometriotic lesions, nanoparticles are constructed to be non-fluorescent prior to internalization by endometriosis cells. In vitro studies confirm that these nanoparticles activate the fluorescence signal following internalization in macaque endometrial stromal cells and ablate them by increasing cellular temperature to 53 ° C upon interaction with NIR light. To demonstrate in vivo efficiency of the nanoparticles, biopsies of endometrium and endometriosis from rhesus macaques are transplanted into immunodeficient mice. Imaging with the intraoperative Fluobeam 800 system reveals that 24 h following intravenous injection, nanoparticles efficiently accumulate in, and demarcate, endometriotic grafts with fluorescence. Finally, the nanoparticles increase the temperature of endometriotic grafts up to 47 °C upon exposure to NIR light, completely eradicating them after a single treatment.

中文翻译:

基于纳米粒子的子宫内膜异位症可激活荧光成像和光热消融平台。

子宫内膜异位症是一种疼痛性疾病,其中子宫内膜样组织在子宫腔外形成病变。术中识别和切除这些病变很困难。这项研究提出了一个纳米平台,它使用实时近红外 (NIR) 荧光和光热疗法 (PTT) 同时描绘和消融子宫内膜异位组织。该纳米平台由一种染料硅萘菁 (SiNc) 组成,它能够进行近红外荧光成像和 PTT,以及一种聚合物纳米颗粒作为 SiNc 载体在全身给药后输送到子宫内膜异位组织。为了在子宫内膜异位病变的荧光成像过程中实现高对比度,纳米颗粒在被子宫内膜异位细胞内化之前被构建为非荧光的。体外研究证实,这些纳米颗粒在猕猴子宫内膜间质细胞内化后激活荧光信号,并通过与 NIR 光相互作用将细胞温度升高至 53°C 来消融它们。为了证明纳米粒子的体内效率,将来自恒河猴的子宫内膜和子宫内膜异位症的活检移植到免疫缺陷小鼠体内。使用术中 Fluobeam 800 系统成像显示,静脉注射后 24 小时,纳米颗粒有效地积聚在子宫内膜异位移植物中并用荧光标定。最后,纳米粒子在暴露于 NIR 光后将子宫内膜异位移植物的温度提高至 47°C,在单次处理后将其完全根除。
更新日期:2020-04-06
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