Novel bifunctional group monomer itaconic acid (IA) based molecularly imprinted polymers (MIPs) were successfully prepared with hydrogen bonding interactions by applying a facile combination of a reversible addition chain transfer mechanism (RAFT) and surface plasmon resonance (SPR). UV photo-polymerization in synergy with 2-methyl-2-[(dodecylsulfanylthiocarbonyl)sulfanyl]propanoic acid (TTCA) as a chain transfer reagent was employed for MIP film synthesis on an SPR sensor chip for the detection of progesterone using ethylene glycol dimethacrylate (EGDMA) as a cross linker. In addition, the ratio of template to bifunctional group monomer to cross linker was optimized (1 : 2 : 12) by real-time SPR monitoring. The modified surface of the biosensor was characterized by contact angle measurements, frontier transfer infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The resulting itaconic acid based MIP (IA based MIP) biosensor showed a high adsorption capacity and excellent selectivity in comparison to other analogues and non-imprinted polymer (NIP) film. Through 8 adsorption–desorption cycles, the high recoverability of IA based MIP film was confirmed. These features suggested that IA based MIPs are ideal candidates for biosensor use owing to the bifunctionality of the monomer. Consequently, within the concentration range of 1 × 10⁻¹⁸ to 1 × 10⁻⁸ mol L⁻¹, the coupling angle change of SPR versus the negative logarithm of concentration showed excellent linearity: R² = 0.99. Based on a linear equation, IA based MIPs showed excellent values for the limit of detection (LOD) and limit of quantification (LOQ), i.e. 0.28 × 10⁻¹⁹ mol L⁻¹ and 0.92 × 10⁻¹⁹ mol L⁻¹, respectively. Furthermore, the influence of the matrix on the biosensor was successfully analyzed in real samples with satisfactory outcomes, displaying identical behavior to ideal samples. Hence, IA based MIPs in combination with an SPR sensor chip demonstrated potential applications for rapid and highly effective sensing even in complicated matrices.

中文翻译：

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新型双官能团单体基分子印迹聚合物在紫外线中仿生检测孕酮

通过应用可逆加成链转移机制（RAFT）和表面等离振子共振（SPR）的简便组合，成功地通过氢键相互作用成功制备了基于双官能团单体衣康酸（IA）的分子印迹聚合物（MIP）。以2-甲基-2-[（十二烷基硫烷基硫代羰基硫烷基）硫烷基]硫烷基]丙酸（TTCA）为链转移剂的紫外光聚合用于SPR传感器芯片上的MIP膜合成，用于使用乙二醇二甲基丙烯酸酯检测孕酮（ EGDMA）作为交叉链接器。此外，通过实时SPR监控优化了模板与双官能团单体与交联剂的比例（1：2：12）。生物传感器的改性表面通过接触角测量来表征，前沿转移红外光谱（FTIR）和扫描电子显微镜（SEM）。与其他类似物和非印迹聚合物（NIP）膜相比，所得衣康酸基MIP（IA基MIP）生物传感器显示出高吸附能力和出色的选择性。通过8个吸附-解吸循环，证实了基于IA的MIP膜的高回收率。这些特征表明基于IA的MIP由于单体的双功能性而成为生物传感器使用的理想候选者。因此，在1×10的浓度范围内 证实了基于IA的MIP膜的高可回收性。这些特征表明基于IA的MIP由于单体的双功能性而成为生物传感器使用的理想候选者。因此，在1×10的浓度范围内 证实了基于IA的MIP膜的高可回收性。这些特征表明基于IA的MIP由于单体的双功能性而成为生物传感器使用的理想候选者。因此，在1×10的浓度范围内^-18至1×10 ^-8 mol L ^-1，SPR的耦合角变化与浓度的负对数表现出极好的线性： R ² = 0.99。基于线性方程，基于IA的MIP在检测限（LOD）和定量限（LOQ）方面显示出极好的值，即0.28×10 ^-19 mol L ^-1和0.92×10 ^-19 mol L ^-1， 分别。此外，在真实样品中成功分析了基质对生物传感器的影响，并获得令人满意的结果，显示出与理想样品相同的行为。因此，基于IA的MIP与SPR传感器芯片的结合展示了即使在复杂的矩阵中也可实现快速，高效感测的潜在应用。