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Bacteria poration on modified boron-doped diamond electrode surfaces induced by divalent cation chelation
Environmental Science: Water Research & Technology ( IF 5 ) Pub Date : 2020-04-06 , DOI: 10.1039/c9ew01108k
Meng-Hsuan Lin 1, 2, 3, 4 , Shafigh Mehraeen 1, 2, 3, 4 , Gang Cheng 1, 2, 3, 4 , Cory Rusinek 4, 5, 6 , Brian P. Chaplin 1, 2, 3, 4
Affiliation  

This research investigated mechanisms for biofouling control at modified boron-doped diamond (BDD) electrode surfaces polarized at low applied potentials (e.g., −0.2 to 1 V versus Ag/AgCl), using Pseudomonas aeruginosa (PAO1) as a model pathogenic organism. Results indicated that electrostatic interactions and electrochemical reactions under polarized conditions can affect cell attachment and poration, respectively. However, results suggested that divalent ions from the outer membrane of PAO1 can be chelated by N-propyl-2-hydroxyacetamide functional groups that were immobilized on a BDD optically transparent electrode (termed OH-BDD/OTE). It was observed that two- to three-fold higher percentage of porated bacteria were observed on the OH-BDD/OTE compared with BDD/OTE under applied anodic potentials between 0.1 to 0.5 V vs. Ag/AgCl. Density functional theory calculations indicated that the chelation mechanism was thermodynamically favorable. Zeta potential measurements of the PAO1 bacteria as a function of chelator and Mg2+ concentrations were performed and interpreted using a mathematical model based on the nonlinear Poisson–Boltzmann equation. Results supported the chelation mechanism for bacteria poration, which indicates that electrode modification may be a viable method to prevent biofouling of electrode surfaces that are operated at low applied potentials.

中文翻译:

二价阳离子螯合在修饰的掺硼金刚石电极表面上的细菌渗透

这项研究使用铜绿假单胞菌(PAO1)作为模型致病生物,研究了在低施加电势(例如-0.2至1 VAg / AgCl)下极化的改性硼掺杂金刚石(BDD)电极表面的生物污染控制机理。结果表明,极化条件下的静电相互作用和电化学反应分别会影响细胞的附着和渗透。然而,结果表明,PAO1外膜上的二价离子可被N螯合固定在BDD光学透明电极上的-丙基-2-羟基乙酰胺官能团(称为OH-BDD / OTE)。观察到在相对于Ag / AgCl的0.1V至0.5V之间施加的阳极电势下,与BDD / OTE相比,在OH-BDD / OTE上观察到的穿孔细菌百分比高出2-3倍。密度泛函理论计算表明,螯合机理在热力学上是有利的。PAO1细菌的Zeta电位测量值与螯合剂和Mg 2+的关系使用基于非线性Poisson–Boltzmann方程的数学模型进行浓度分析。结果支持了细菌渗透的螯合机制,这表明电极修饰可能是防止在低施加电势下操作的电极表面发生生物结垢的可行方法。
更新日期:2020-04-06
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