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Genomic and Phenotypic Analyses of Acinetobacter baumannii Isolates From Three Tertiary Care Hospitals in Thailand.
Frontiers in Microbiology ( IF 5.2 ) Pub Date : 2020-04-06 , DOI: 10.3389/fmicb.2020.00548
Jessica Loraine 1 , Eva Heinz 2, 3 , Rosesathorn Soontarach 4 , Grace A Blackwell 3, 5 , Richard A Stabler 6 , Supayang P Voravuthikunchai 4 , Potjanee Srimanote 7 , Pattarachai Kiratisin 8 , Nicholas R Thomson 3, 6 , Peter W Taylor 1
Affiliation  

Antibiotic resistant strains of Acinetobacter baumannii are responsible for a large and increasing burden of nosocomial infections in Thailand and other countries of Southeast Asia. New approaches to their control and treatment are urgently needed and an attractive strategy is to remove the bacterial polysaccharide capsule, and thus the protection from the host's immune system. To examine phylogenetic relationships, distribution of capsule chemotypes, acquired antibiotic resistance determinants, susceptibility to complement and other traits associated with systemic infection, we sequenced 191 isolates from three tertiary referral hospitals in Thailand and used phenotypic assays to characterize key aspects of infectivity. Several distinct lineages were circulating in three hospitals and the majority belonged to global clonal group 2 (GC2). Very high levels of resistance to carbapenems and other front-line antibiotics were found, as were a number of widespread plasmid replicons. A high diversity of capsule genotypes was encountered, with only three of these (KL6, KL10, and KL47) showing more than 10% frequency. Almost 90% of GC2 isolates belonged to the most common capsule genotypes and were fully resistant to the bactericidal action of human serum complement, most likely protected by their polysaccharide capsule, which represents a key determinant of virulence for systemic infection. Our study further highlights the importance to develop therapeutic strategies to remove the polysaccharide capsule from extensively drug-resistant A. baumanii during the course of systemic infection.

中文翻译:

来自泰国三家三级医院的鲍曼不动杆菌分离株的基因组和表型分析。

鲍曼不动杆菌的抗药性菌株在泰国和东南亚其他国家造成医院感染的巨大且日益增加的负担。迫切需要新的控制和治疗方法,一种有吸引力的策略是去除细菌多糖胶囊,从而保护其免受宿主免疫系统的侵害。为了检查系统发育关系,胶囊化学型分布,获得性抗生素耐药性决定因素,对补体的易感性以及与系统感染相关的其他特征,我们对来自泰国三所转诊医院的191株分离株进行了测序,并使用表型分析来表征传染性的关键方面。几家不同的血统在三家医院中流传,大多数属于全球性克隆组2(GC2)。发现对碳青霉烯类和其他一线抗生素的抗药性很高,还有许多广泛的质粒复制子。胶囊基因型的多样性很高,只有其中三种(KL6,KL10和KL47)显示出超过10%的频率。几乎90%的GC2分离物属于最常见的胶囊基因型,并且完全抵抗人血清补体的杀菌作用,很可能受到其多糖胶囊的保护,而多糖胶囊代表了全身感染毒力的关键决定因素。我们的研究进一步强调了开发治疗策略以在全身感染过程中从广泛耐药的鲍曼不动杆菌中除去多糖胶囊的重要性。以及许多广泛的质粒复制子。胶囊基因型的多样性很高,只有其中三种(KL6,KL10和KL47)显示出超过10%的频率。几乎90%的GC2分离物属于最常见的胶囊基因型,并且完全抵抗人血清补体的杀菌作用,很可能受到其多糖胶囊的保护,而多糖胶囊代表了全身感染毒力的关键决定因素。我们的研究进一步强调了开发治疗策略以在全身感染过程中从广泛耐药的鲍曼不动杆菌中除去多糖胶囊的重要性。以及许多广泛的质粒复制子。胶囊基因型的多样性很高,只有其中三种(KL6,KL10和KL47)显示出超过10%的频率。几乎90%的GC2分离物属于最常见的胶囊基因型,并且完全抵抗人血清补体的杀菌作用,很可能受到其多糖胶囊的保护,而多糖胶囊代表了全身感染毒力的关键决定因素。我们的研究进一步强调了开发治疗策略以在全身感染过程中从广泛耐药的鲍曼不动杆菌中除去多糖胶囊的重要性。几乎90%的GC2分离物属于最常见的胶囊基因型,并且完全抵抗人血清补体的杀菌作用,很可能受到其多糖胶囊的保护,而多糖胶囊代表了全身感染毒力的关键决定因素。我们的研究进一步强调了开发治疗策略以在全身感染过程中从广泛耐药的鲍曼不动杆菌中除去多糖胶囊的重要性。几乎90%的GC2分离物属于最常见的胶囊基因型,并且完全抵抗人血清补体的杀菌作用,很可能受到其多糖胶囊的保护,而多糖胶囊代表了全身感染毒力的关键因素。我们的研究进一步强调了开发治疗策略以在全身感染过程中从广泛耐药的鲍曼不动杆菌中除去多糖胶囊的重要性。
更新日期:2020-04-08
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