当前位置: X-MOL 学术Mol. Biol. Rep. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Advances in diagnosis of Tuberculosis: an update into molecular diagnosis of Mycobacterium tuberculosis.
Molecular Biology Reports ( IF 2.8 ) Pub Date : 2020-04-04 , DOI: 10.1007/s11033-020-05413-7
Bodhraj Acharya 1 , Ashma Acharya 2 , Sanjay Gautam 3 , Sagar Prasad Ghimire 4 , Gokul Mishra 5 , Navin Parajuli 6 , Bishwa Sapkota 7
Affiliation  

Tuberculosis (TB) is a major cause of deaths by a single infectious agent and has now been a global public health problem due to increasing numbers of drug-resistant cases. Early and effective treatment is crucial to prevent the emergence of drug-resistance strains. This demands the availability of fast and reliable point-of-care (POC) diagnostic methods for effective case management. Commonly used methods to screen and diagnose TB are clinical, immunological, microscopy, radiography, and bacterial culture. In addition, recent advances in molecular diagnostic methods including MTBDRplus, loop-mediated isothermal amplification (LAMP), line probe assay (LPA), GeneXpert, and whole genome sequencing (WGS) have been employed to diagnose and characterize TB. These methods can simultaneously identify Mycobacterium tuberculosis (MTB) and mutation(s) associated with routinely used anti-TB drugs. Here, we review the use of currently available diagnostic methods and strategies including conventional to recently implemented next-generation sequencing (NGS) methods used to detect MTB in clinical perspective.

中文翻译:

结核病诊断进展:结核分枝杆菌分子诊断的最新进展。

结核病(TB)是单一传染病致死的主要原因,由于耐药病例的增加,现已成为全球公共卫生问题。早期有效的治疗对防止耐药菌株的出现至关重要。这就要求提供快速,可靠的即时护理(POC)诊断方法,以进行有效的病例管理。筛查和诊断结核病的常用方法是临床,免疫学,显微镜检查,放射线照相术和细菌培养。此外,分子诊断方法的最新进展已用于诊断和表征结核病,包括MTBDRplus,环介导的等温扩增(LAMP),线探针测定(LPA),GeneXpert和全基因组测序(WGS)。这些方法可同时识别结核分枝杆菌(MTB)和与常规使用的抗结核药物相关的突变。在这里,我们从临床角度回顾了当前可用的诊断方法和策略的使用,包括常规的到最近实施的下一代测序(NGS)方法,这些方法用于检测MTB。
更新日期:2020-04-06
down
wechat
bug